Extraction and protein component analysis of venom from the dissected venom glands of Latrodectus tredecimguttatus
Extraction and protein component analysis of venom from the dissected venom glands of Latrodectus tredecimguttatus
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Latrodectus tredecimguttatus 毒腺解剖毒液的提取及蛋白质成分分析
DOI：
10.1016/j.cbpb.2006.08.006
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发表时间：
2006-11-01
影响因子：
2.2
通讯作者：
Liang, S. P.
中科院分区：
文献类型：
--
作者：
Duan, Z. G.;Yan, X. J.;Liang, S. P.
黑寡妇蜘蛛（Latrodectus）因其对人类的伤害和其毒液中生物活性成分的潜在应用而受到越来越多的关注。虽然许多研究已经描述了几种有毒蛋白质的生物学特性和结构，如latrotoxins，但对蜘蛛毒液的蛋白质组分的全面分析尚不存在。我们使用组合的蛋白质组学策略，以评估粗毒液的蛋白质组分，从Latrodectus tredecimguttatus通过提取解剖的毒腺。实验证明，L. tredecimguttatus具有高丰度的分子量大于15 kDa的酸性蛋白质，并且低于15 kDa的蛋白质和肽的含量低。鉴定了86种独特的蛋白质，其中一部分是提取过程中细胞成分的污染，与通过电刺激获得的毒液进行比较。除主要毒性成分为latrotoxin家族成员外，还检测到蛋白酶、磷酸酶、溶菌酶、抑制蛋白等多种特异性酶和蛋白质，推测这些蛋白质组分，尤其是蛋白酶在L.刺尾蛇毒液(c)2006年爱思唯尔公司All rights reserved.
Black widow spiders (genus Latrodectus) have attracted increasing attention due to frequently reported human injuries caused by them and the potential applications of biologically active components in their venoms. Although a number of studies have described the biological properties and structures of several venomous proteins such as latrotoxins, a comprehensive analysis of protein component of the venom from the spider is not available. We used combinative proteomic strategies to assess the protein components of the crude venom collected from Latrodectus tredecimguttatus by extracting the dissected venom glands. The experiments demonstrated that the crude venom of L. tredecimguttatus has a high abundance of acidic proteins with molecular masses greater than 15 kDa, and the content of proteins and peptides of below 15 kDa is low. 86 unique proteins were identified, part of which were contaminations of cellular components during the extraction, determined in comparison with venom obtained by electrostimulation. Except for members of latrotoxin family that were commonly considered as the primary toxic components of the venom, several other special enzymes and proteins were detected such as protease, phosphatase, lysozyme, inhibitory protein, and so on. These protein components, particularly the proteases, were speculated to play important roles in the action of L. tredecimguttatus venom. (c) 2006 Elsevier Inc. All rights reserved.