Mutation of CYCLOIDEA Expands Variation of Dorsal–ventral Flower Asymmetry Expressed as a Pigmentation Pattern in Torenia fournieri Cultivars
Mutation of CYCLOIDEA Expands Variation of Dorsal–ventral Flower Asymmetry Expressed as a Pigmentation Pattern in Torenia fournieri Cultivars
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DOI：
10.2503/hortj.utd-174
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
M. Kondo;N. Tanikawa;T. Nishijima
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文献类型：
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作者：
M. Kondo;N. Tanikawa;T. Nishijima
我们研究了蓝猪耳 (Torenia fournieri Lind. ex Fourn.) 品种背面花瓣色素沉着模式的分子机制。 “Piccolo Mix”由背瓣边缘表现出不同色素沉着模式的线条组成，即完全着色（全着色线）、带有着色边缘的小部分（半着色线）和完全苍白（苍白线）。在全色素系和半色素系中，T. fournieri CYCLOIDEA2 ( TfCYC2 ) 的表达（参与背腹花不对称）通过将 Ty1/Copia 样 LTR 逆转录转座子 TORE2 整合到 TfCYC2 ( TfCYC2 TORE2 ) 的外显子中而受到抑制。全色素系是TfCYC2 TORE2 纯合的，而浅色系是纯合正常型TfCYC2 (TfCYC2 + )的。半色素系是杂合的TfCYC2 TORE2 / TfCYC2 + 。因此，背花瓣色素沉着程度与TfCYC2 + 基因剂量呈负相关。此外，另一种蓝猪草品种“Crown Violet”与“Piccolo Mix”的全色素系表现出相同的色素沉着模式，对于 TfCYC2 TORE2 也是纯合的。这些结果表明，TfCYC2 TORE2 负责蓝猪耳品种中花朵色素沉着模式的显着富集。
We investigated the molecular mechanisms underlying the pigmentation patterns of the dorsal petals in torenia ( Torenia fournieri Lind. ex Fourn.) cultivars. ‘Piccolo Mix’ consists of lines exhibiting different pigmentation patterns in the limb of the dorsal petal, i.e., entirely pigmented (all-pigmented line), picotee with a pigmented margin (half-pigmented line), and entirely pale (pale line). In the all- and half-pigmented lines, expression of T. fournieri CYCLOIDEA2 ( TfCYC2 ), which is involved in dorsal–ventral floral asymmetry, was inhibited by integration of Ty1/Copia-like LTR retrotransposon TORE2 into the exon of TfCYC2 ( TfCYC2 TORE2 ). The all-pigmented line was homozygotic for TfCYC2 TORE2 , while the pale line was homozygotic normal-type TfCYC2 ( TfCYC2 + ). The half-pigmented line was heterozygotic TfCYC2 TORE2 / TfCYC2 + . Therefore, the extent of pigmentation of the dorsal petal is negatively correlated with the gene dosage of TfCYC2 + . Further, ‘Crown Violet’, another torenia cultivar exhibiting the same pigmentation pattern as the all-pigmented line of ‘Piccolo Mix’, was also homozygotic for TfCYC2 TORE2 . These results indicate that TfCYC2 TORE2 is responsible for the marked enrichment of flower pigmentation patterns seen in torenia cultivars.