Evaluation of Antioxidant Activity of Natural and Synthetic Compounds Against LDL oxidation Using CNT electrode
Evaluation of Antioxidant Activity of Natural and Synthetic Compounds Against LDL oxidation Using CNT electrode
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使用 CNT 电极评价天然和合成化合物抗 LDL 氧化的抗氧化活性
DOI：
10.1109/jsen.2013.2285408
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发表时间：
2014
影响因子：
4.3
通讯作者：
Hitoshi Chiba
中科院分区：
文献类型：
--
作者：
Futaba Ohkawa;Seiji Takeda;Shu-Ping Hui;Toshihiro Sakurai;Shigeki Jin;Hirotoshi Fuda;Kazuhisa Sueoka;Hitoshi Chiba
研究了一种利用碳纳米管（CNT）电极评价低密度脂蛋白（LDL）氧化（anti-LDLox）的新方法。虽然ORAC和DPPH是已知的评价抗氧化活性的方法，但它们不能直接反映抗LDLox的能力。已经报道了使用CNT电极测量氧化LDL的方法。我们通过在铜介导的氧化过程中将LDL与抗氧化剂孵育后使用电极测量氧化LDL的量来评估抗LDLox。还测定了硫代巴比妥酸反应物质（TBARS）以与CNT电极进行比较。碳纳米管电极与TBARS和ORAC之间存在相关性，而碳纳米管电极与DPPH之间没有发现相关性。我们在这里证明，碳纳米管电极方法有可能通过检测氧化的低密度脂蛋白来测定各种天然和合成化合物的抗氧化活性。
In this paper, a novel method for evaluating antioxidant activity against low density lipoprotein (LDL) oxidation (anti-LDLox) using a carbon nanotube (CNT) based electrode was investigated. Although ORAC and DPPH are known methods to evaluate antioxidant activity, they do not directly reflect the capacities of anti-LDLox. A method has been reported for measuring oxidized LDL using a CNT electrode. We evaluated anti-LDLox by measuring the amount of oxidized LDL using the electrode after incubation of LDL with antioxidants during copper-mediated oxidation. Thiobarbituric acid reactive substances (TBARS) were also determined for comparison with the CNT electrode. There was a correlation of the CNT electrode with the TBARS and the ORAC, whereas no correlation was found between the CNT electrode and the DPPH. We demonstrate here that a CNT electrode method has a potential for the determination of the antioxidant activity of various natural and synthetic compounds by detecting oxidized LDL.