Femoral Artery Occlusion Increases Muscle Pressor Reflex and Expression of Hypoxia-Inducible Factor-1α in Sensory Neurons.
Femoral Artery Occlusion Increases Muscle Pressor Reflex and Expression of Hypoxia-Inducible Factor-1α in Sensory Neurons.
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发表时间：
2013-10
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通讯作者：
W. Gao;Jianhua Li
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作者：
W. Gao;Jianhua Li
缺氧诱导因子-1（Hypoxia inducible factor-1，HIF-1）在缺氧和缺血条件下对体内平衡的病理生理变化具有重要作用。观察股动脉阻断对大鼠背根神经节（DRG）感觉神经元缺氧诱导因子-1 α（HIF-1α）表达的影响。此外，我们研究了股动脉闭塞后静态肌肉收缩的心血管反应。我们假设后肢血管功能不全增加了感觉神经的HIF-1α水平，并增强了由肌肉传入神经激活引起的自主反应。此外，我们还检测了当DRG神经元中HIF-1α增加时，反射性心血管反应是否改变。结果表明，股动脉结扎后6、24和72小时，腰段背根节神经元中HIF-1α的表达明显高于假手术组。给予HIF-α稳定剂二甲基草酰甘氨酸（DMOG）显著增加腰DRG神经元中的HIF-1α。此外，股动脉闭塞增强了反射性升压反应肌肉收缩，然而，该反应并没有改变注射DMOG。总之，我们的研究结果表明：1）股动脉闭塞增加DRG神经元中HIF-1α的水平和收缩诱导的升压反应; 2）DRG神经元本身HIF-1α的增加可能不改变肌肉升压反射。
Hypoxia inducible factor-1 (HIF-1) has an important contribution to pathophysiological changes of homeostasis under conditions of oxygen deprivation as well as ischemia. We examined the effects of femoral artery occlusion on HIF-1α expression in sensory dorsal root ganglion (DRG) neurons of rats. Also, we examined cardiovascular responses to static muscle contraction following femoral occlusion. We hypothesized that hindlimb vascular insufficiency increases the levels of sensory nerves' HIF-1α and augments autonomic responses induced by activation of muscle afferent nerves. In addition, we examined if the reflex cardiovascular responses were altered as HIF-1α was increased in the DRG neurons. Our data show that HIF-1α was significantly increased in the lumbar DRG neurons 6, 24 and 72 hours after femoral artery ligation as compared with sham control. Administration of dimethyloxalylglycine (DMOG), a stabilizer of HIF-α, significantly increased HIF-1α in the lumbar DRG neurons. Furthermore, femoral occlusion enhanced the reflex pressor response to muscle contraction; however, the response was not altered by injection of DMOG. Overall, our results indicate that 1) femoral artery occlusion increases HIF-1α levels of in DRG neurons and contraction-induced pressor response; and 2) an increase in HIF-1α of DRG neurons per se may not alter the muscle pressor reflex.