flp gene disruption in a parasitic nematode reveals motor dysfunction and unusual neuronal sensitivity to RNA interference
flp gene disruption in a parasitic nematode reveals motor dysfunction and unusual neuronal sensitivity to RNA interference
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DOI：
10.1096/fj.06-7343com
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发表时间：
2007-04-01
期刊：
影响因子：
4.8
通讯作者：
Maule, Aaron G.
中科院分区：
文献类型：
--
作者：
Kimber, Michael J.;McKinney, Susan;Maule, Aaron G.
马铃薯孢囊线虫Globodera pallida是马铃薯作物的严重害虫。线虫FMRFamide-like peptides（FLP）是已知的最多样化的神经肽家族之一，并且调节感觉和运动功能。由于神经肌肉功能是寄生虫控制的既定目标，寄生线虫FLP信号传导在新的控制策略中具有重要的潜力。在转基因寄生线虫的情况下，以及报道的秀丽隐杆线虫中神经元基因RNAi的无效性，对线虫寄生虫中flp的功能一无所知。为了评价flp在G. pallida，我们发现，与C.这些基因很容易受到RNAi的影响。沉默G.苍白球flp基因（Gp-flp-1、-6、-12、-14或-18）引起与运动功能中的关键作用一致的不同的异常行为表型。对这些效应的进一步描述表明，双链RNA暴露时间（>= 18小时）和浓度（>= 0.1 μ g/ml）对观察到的效应至关重要，这些效应是可逆的。G.苍白球flp基因是协调运动活动所必需的，不显示冗余，并且对RNAi敏感，为研究RNAi介导的flp基因沉默作为一种新的植物寄生虫控制策略铺平了道路。
The potato cyst nematode Globodera pallida is a serious pest of potato crops. Nematode FMRFamide-like peptides (FLPs) are one of the most diverse neuropeptide families known, and modulate sensory and motor functions. As neuromuscular function is a well-established target for parasite control, parasitic nematode FLP signaling has significant potential in novel control strategies. In the absence of transgenic parasitic nematodes and the reported ineffectiveness of neuronal gene RNAi in Caenorhabditis elegans, nothing is known about flp function in nematode parasites. In attempts to evaluate flp function in G. pallida, we have discovered that, unlike in C. elegans, these genes are readily susceptible to RNAi. Silencing any of the five characterized G. pallida flp genes (Gp-flp-1, -6, -12, -14, or -18) incurred distinct aberrant behavioral phenotypes consistent with key roles in motor function. Further delineation of these effects revealed that double-stranded RNA exposure time (>= 18 h) and concentration (>= 0.1 mu g/ml) were critical to the observed effects, which were reversible. G. pallida flp genes are essential to coordinated locomotory activities, do not display redundancy, and are susceptible to RNAi, paving the way for the investigation of RNAi-mediated flp gene silencing as a novel plant parasite control strategy.