Rat mast cells communicate with fibroblasts via gap junction intercellular communications
Rat mast cells communicate with fibroblasts via gap junction intercellular communications
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DOI：
10.1002/jcb.21107
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发表时间：
2007-04-01
影响因子：
4
通讯作者：
Ehrlich, H. Paul
中科院分区：
文献类型：
--
作者：
Au, Stephen R.;Au, Katherine;Ehrlich, H. Paul
肥大细胞（MC）通常通过释放其胞质颗粒来调节其他细胞活动。最近，间隙连接细胞间通讯（GJIC）之间建立的人MC细胞系（HMC-1）与人真皮成纤维细胞在成纤维细胞填充的胶原晶格（FPCL）共培养，提高FPCL收缩的速度和程度。然而，在单层培养中，HMC-1细胞不能与人新生儿成纤维细胞产生GJIC。在这里，新鲜分离的大鼠腹膜MCs与成纤维细胞在胶原晶格中共培养，并在体外单层培养，并在体内引入大鼠聚乙烯醇（PVA）海绵植入物。共培养的MC-FPCL收缩更快，程度更大。将钙黄绿素AM绿色荧光染料装载到红色荧光Dil标记的MC中产生MC-伞兵。当MC-伞兵与成纤维细胞形成GJIC时，一些绿色染料被传递到成纤维细胞中，而MC-伞兵保留其红色和绿色荧光。MC-伞兵通过绿色荧光染料进入单层培养的人和大鼠皮肤成纤维细胞。在大鼠7天大的皮下PVA海绵植入物，其中接受了MC-伞兵注射，表现出自发荧光绿色成纤维细胞，当收获24小时后。MC-伞兵预处理的长效GJIC抑制剂之前，他们引入PVA海绵植入物，未能通过染料成纤维细胞。提出肉芽组织成纤维细胞和MC之间的GJIC可以调节创伤修复和纤维化的某些方面。
Usually mast cells (MCs) modulate other cellular activities through the release of their cytoplasmic granules. Recently, gap junctional intercellular communication (GJIC) between an established human MC cell line (HMC-1) co-cultured with human dermal fibroblasts in fibroblast populated collagen lattices (FPCLs), enhanced the rate and degree of FPCL contraction. However, HMC-1 cells were unable to generate GJIC with human neonatal fibroblasts in monolayer culture. Here freshly isolated rat peritoneal MCs are co-cultured with fibroblasts in collagen lattices and in monolayer culture in vitro and introduced into rat polyvinyl alcohol (PVA) sponge implants in vivo. Co-cultured MC-FPCL contracted faster and to a greater degree. Loading Calcein AM green fluorescent dye into red fluorescent Dil tagged MC generates MC-paratroopers. When MC-paratroopers form GJIC with fibroblasts, some green dye is passed into the fibroblast, while the MC-paratrooper retains both its red and green fluorescence. MC-paratroopers passed green fluorescent dye into both human and rat dermal fibroblasts in monolayer culture. In rats 7-day-old subcutaneous PVA sponge implants, which received an injection of MC-paratroopers, exhibited auto-fluorescent green fibroblasts, when harvested 24 h later. MC-paratroopers pretreated with a long-acting GJIC inhibitor prior to their introduction into PVA sponge implants, failed to pass dye into fibroblasts. it is proposed that GJIC between granulation tissue fibroblasts and MCs can modulate some aspects of wound repair and fibrosis.