Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.
Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.
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荧光微量法测定滤纸上干血斑中的精氨酸酶活性。
DOI：
10.1093/clinchem/26.8.1198
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发表时间：
1980
影响因子：
9.3
通讯作者：
R. Guthrie
中科院分区：
文献类型：
--
作者：
A. Orfanos;E. Naylor;R. Guthrie
我们描述了一种测定滤纸上干血斑点精氨酸酶活性的微荧光法。在37℃下用Mn2+预孵育激活从这些干燥的血液标本中打出来的圆盘中的精氨酸酶。在相同温度下与底物孵育后，通过耦合动力学反应将NADH氧化为NAD+来荧光测定尿素。我们将这种方法的结果与涉及液体血液样本的比色法的结果进行比较，并评估滤纸上干血中酶的稳定性。血清的存在对活性没有影响。该方法可用于精氨酸酶缺乏和某些血液学疾病的早期检测。
We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.