Determination of saponins in legumes by direct densitometry
Determination of saponins in legumes by direct densitometry
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DOI：
10.1021/jf010754c
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发表时间：
2002-01-30
影响因子：
6.1
通讯作者：
Rao, AV
中科院分区：
文献类型：
--
作者：
Gurfinkel, DM;Rao, AV
Research has shown that dietary saponins may have health benefits. A simple, rapid method for the determination of saponins in legumes, using densitometry, is described. Saponin preparations, after pretreatment to remove nonsaponin components, are spotted in rows on a thin-layer chromatography plate, along with soyasaponin standards. The plate, without solvent development, is directly treated with sulfuric acid and heated. Violet spots develop which have a density proportional to the amount of saponin present. The standard curve has a correlation coefficient of 0.99 and is linear over the range of 1.25 to 10 mug of soyasaponins applied. The method has a coefficient of variation of less than 3% and compares favorably with quantitative thin-layer chromatography. Using this method the saponin contents of defatted soy flour (0.58%), dried navy beans (0.32%), and dried kidney beans (0.29%) were determined, and these results were found to be consistent with previous reports in the literature.
