Caspase-8 and Caspase-9 Functioned Differently at Different Stages of the Cyclic Stretch-Induced Apoptosis in Human Periodontal Ligament Cells.
Caspase-8 and Caspase-9 Functioned Differently at Different Stages of the Cyclic Stretch-Induced Apoptosis in Human Periodontal Ligament Cells.
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Caspase-8和caspase-9在人牙周膜细胞循环拉伸诱导细胞凋亡的不同阶段发挥不同的作用
DOI：
10.1371/journal.pone.0168268
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Wu Y;Zhao D;Zhuang J;Zhang F;Xu C
人牙周膜细胞在机械牵张负荷后发生凋亡。然而，确切的信号通路仍然未知。本研究旨在阐明凋亡半胱氨酸蛋白酶在周期性牵张诱导人牙周膜细胞凋亡中的作用。在本研究中，选择20%的周期性拉伸来加载细胞6或24 h。检测细胞凋亡率、caspase-3、caspase-7、caspase-8、caspase-9蛋白水平及caspase-8、caspase-9活性。随后，还评估了caspase-8和caspase-9抑制剂对凋亡率和活化的caspase-3蛋白水平的影响。周期性牵张使细胞凋亡率增加，但牵张6 h和24 h后细胞进入不同的凋亡阶段。Caspase-3、Caspase-7、Caspase-8和Caspase-9在拉伸负荷后均被激活。在拉伸6和24 h的细胞中抑制caspase-8和caspase-9以及在拉伸24 h的细胞中抑制caspase-9后，拉伸诱导的凋亡和活化的caspase-3的蛋白水平被抑制。Caspase-8和-9在周期性牵张后人PDL细胞凋亡的不同阶段发挥不同的作用。
Human periodontal ligament (PDL) cells underwent apoptosis after mechanical stretch loading. However, the exact signalling pathway remains unknown. This study aimed to elucidate how the apoptotic caspases functioned in the cyclic stretch-induced apoptosis in human PDL cells. In the present study, 20% cyclic stretch was selected to load the cells for 6 or 24 h. The following parameters were analyzed: apoptotic rates, the protein levels of caspase-3, -7, -8 and -9 and the activities of caspase-8 and -9. Subsequently, the influences of caspase-8 and caspase-9 inhibitors on the apoptotic rate and the protein level of the activated caspase-3 were assessed as well. The apoptotic rates increased in response to cyclic stretch, but the cells entered different apoptotic stages after 6 and 24 h stretches. Caspase-3, -7, -8 and -9 were all activated after stretch loading. The stretch-induced apoptosis and the protein level of the activated caspase-3 were inhibited after inhibiting both caspase-8 and caspase-9 in both 6 and 24 h stretched cells and after inhibiting caspase-9 in 24 h stretched cells. Caspase-8 and -9 functioned differently at different apoptotic stages in human PDL cells after cyclic stretch.
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