Production and in vivo PET/CT imaging of the theranostic pair 132/135La
Production and in vivo PET/CT imaging of the theranostic pair 132/135La
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DOI：
10.1038/s41598-019-47137-0
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发表时间：
2019-07-23
影响因子：
4.6
通讯作者：
Engle, Jonathan W.
中科院分区：
文献类型：
--
作者：
Aluicio-Sarduy, Eduardo;Hernandez, Reinier;Engle, Jonathan W.
本研究描述了一种低能回旋加速器生产和从大块钡中分离无载流子La-132/135（3+）的新方法。该分离策略结合了沉淀和单柱萃取层析，总体放射化学产率（92 +/- 2%）和表观摩尔活性（22 +/- 4 Mbq/nmol）适合于dota共轭载体的放射性标记。生产的La-132/135（3+）具有放射化学和放射性核素纯度，可用于基于La-132/ la -135的癌症治疗应用。利用正电子发射的La-132纵向PET/CT图像和离体生物分布数据分别证实了未螯合的La-132/135（3+）离子在骨骼和肝脏中的积累。
The present study describes a novel method for the low energy cyclotron production and radiochemical isolation of no-carrier-added La-132/135(3+) from bulk Ba-nat. This separation strategy combines precipitation and single-column extraction chromatography to afford an overall radiochemical yield (92 +/- 2%) and apparent molar activity (22 +/- 4 Mbq/nmol) suitable for the radiolabeling of DOTA-conjugated vectors. The produced La-132/135(3+) has a radiochemical and radionuclidic purity amenable for La-132/La-135-based cancer theranostic applications. Longitudinal PET/CT images acquired using the positron-emitting La-132 and ex vivo biodistribution data separately corroborated the accumulation of unchelated La-132/135(3+) ions in bone and the liver.