Highly effective contact antimicrobial surfaces via polymer surface modifiers
Highly effective contact antimicrobial surfaces via polymer surface modifiers
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DOI：
10.1021/la063718m
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发表时间：
2007-04-24
期刊：
影响因子：
3.9
通讯作者：
Wynne, Kenneth J.
中科院分区：
文献类型：
--
作者：
Kurt, Pinar;Wood, Lynn;Wynne, Kenneth J.
具有聚(烷基铵)组合物的接触式抗菌涂层已成为人们日益关注的主题，部分原因是杀生物剂释放涂层对抗生素耐药性的贡献。本文中，抗菌涂层的概念是基于软嵌段侧链的热力学驱动表面浓度而开发的。该概念结合了天然抗菌蛋白的结构和成分指导，并通过聚合物表面改性剂（PSM）实现成分经济。为了实现这一概念，制备了具有无规共聚物 1,3-环氧丙烷软嵌段和烷基铵以及三氟乙氧基或聚乙二醇化侧链的聚氨酯。使用六碳(C6)和十二碳(C12)烷基铵链长。 PSM 首先作为 100% 涂层进行测试，对于革兰氏阴性菌（铜绿假单胞菌和大肠杆菌）的气溶胶挑战非常有效。为了评估表面浓度，将含有 2 wt% PSM 和常规聚氨酯的溶液蒸发涂覆到载玻片上。这些 2% PSM 涂层针对革兰氏阴性菌（铜绿假单胞菌和大肠杆菌）和革兰氏阳性菌（金黄色葡萄球菌）的气溶胶挑战进行了测试（10(7) CFU/mL/30 分钟）。含有三氟乙氧基（89 mol%）和 C-12 烷基铵（11 mol%）侧链的共聚物软嵌段在 30 分钟内具有最高的杀菌效果：2 wt%，革兰氏（+/-）细菌，100% 杀灭，3.6-4.4 log 减少。抑制区测试显示PSM和PSM改性组合物没有释放杀生物剂。有助于概念验证的特性包括良好的硬嵌段/软嵌段相分离、模拟天然杀生物蛋白的阳离子/共重复基团比率、有助于烷基铵表面浓度的半氟化“伴侣”以及烷基铵软嵌段的低T-g。
Contact antimicrobial coatings with poly(alkylammonium) compositions have been a subject of increasing interest in part because of the contribution of biocide release coatings to antibiotic resistance. Herein, a concept for antimicrobial coatings is developed on the basis of the thermodynamically driven surface concentration of soft block side chains. The concept incorporates structural and compositional guidance from naturally occurring antimicrobial proteins and achieves compositional economy via a polymer-surface modifier (PSM). To implement this concept, polyurethanes were prepared having random copolymer 1,3-propylene oxide soft blocks with alkylammonium and either trifluoroethoxy or PEGlyted side chains. Six carbon (C6) and twelve carbon (C12) alkylammonium chain lengths were used. The PSMs were first tested as 100% coatings and were highly effective against aerosol challenges of Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). To evaluate the surface concentration, solutions containing 2 wt % PSM with a conventional polyurethane were evaporatively coated onto glass slides. These 2% PSM coatings were tested against aerosol challenges of Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria (10(7) CFU/mL/30 min). A copolymer soft block containing trifluorethoxy (89 mol %) and C-12 alkylammonium (11 mol %) side chains gave the highest biocidal effectiveness in 30 min: 2 wt %, Gram(+/-) bacteria, 100% kill, and 3.6-4.4 log reduction. A zone of inhibition test showed no biocide release for PSMs and PSM-modified compositions. Characteristics that contribute to concept validation include good hard block/soft block phase separation, a cation/co-repeat group ratio mimicking natural biocidal proteins, a semifluorinated "chaperone" aiding in alkylammonium surface concentration, and a low T-g for the alkylammonium soft block.