Stomatal behaviors reflect enantioselective phytotoxicity of chiral herbicide dichlorprop in Arabidopsis thaliana
Stomatal behaviors reflect enantioselective phytotoxicity of chiral herbicide dichlorprop in Arabidopsis thaliana
复制标题
拟南芥气孔行为反映了手性除草剂敌敌畏的对映选择性药害
DOI：
10.1016/j.scitotenv.2016.03.205
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发表时间：
2016
影响因子：
9.8
通讯作者：
Wen Yuezhong
中科院分区：
文献类型：
--
作者：
Chen Zunwei;Chen Hui;Zou Yuqin;Wen Yuezhong
植物气孔在植物的水分蒸腾和光合作用所需的气体交换中起着至关重要的作用，对植物的生长至关重要。然而，到目前为止，手性除草剂对气孔反应的影响知之甚少。为了揭开这一谜团，本文研究了手性除草剂敌敌畏（DCPP）对拟南芥气孔的对映体选择性效应。结果表明，（R）-DCPP优先促进水稻叶片气孔的开放，在0.3 μmol·L-1浓度下比对照提高了59.84%，而（S）-DCPP和（Rac）-DCPP的差异不显著。在气孔对DCPP的反应中也观察到对映选择性。为了更好地理解所涉及的机制，测量了活性氧（ROS）的产生和抗氧化系统的防御。有趣的是，ROS的生产在opsisleaves也是对映选择性的。与对照相比，（R）-DCPP处理导致6.08倍的增强，而（S）-DCPP和（Rac）-DCPP处理分别增加1.35倍和2.51倍。气孔开放的促进作用与活性氧的产生呈正相关。此外，抗氧化系统的反应提供了DCPP引起的氧化应激和损伤的证据。本研究证实了DCPP产生的活性氧促进气孔开放，为阐明手性除草剂的植物毒性提供了一个潜在的视角。
Stomata in plants play vital roles in water transpiration and gas exchange necessary for photosynthesis, which are critical for the plants growth. Until now, however, the effect of chiral herbicides on the response of stomata was poorly understood. To unveil this puzzle, the enantioselective effect of chiral herbicide dichloroprop (DCPP) on stomata inArabidopsis thalianawas investigated. It was found that (R)-DCPP preferentially promoted the extent of stomatal opening inArabidopsisleaves, resulting in 59.84% enhancement at 0.3 μmol·L− 1comparing to the control, where (S)- and (Rac)-DCPP exhibited no significant differences. Enantioselectivity was also observed in the response of stomata to DCPP. To better understand the mechanism involved, the reactive oxygen species (ROS) production and antioxidant system defense were measured. Interestingly, the ROS production inArabidopsisleaves was also enantioselective. The (R)-DCPP treatments resulted in 6.08-fold enhancement compared with the control, whereas 1.35- and 2.51-fold increases occurred in (S)-DCPP and (Rac)-DCPP treatments, respectively. The promoting of stomatal opening was positively correlated with ROS production. In addition, the antioxidant system response provided evidence of oxidative stress and damage caused by DCPP. This study confirmed that the ROS produced by DCPP promoted stomatal opening and suggested a potential sight to elucidate the phytotoxicity of chiral herbicides.