Colony variation of Helicobacter pylori: Pathogenic potential is correlated to cell wall lipid composition
Colony variation of Helicobacter pylori: Pathogenic potential is correlated to cell wall lipid composition
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DOI：
10.3109/00365529709025079
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发表时间：
1997-05-01
影响因子：
1.9
通讯作者：
Guldvog, I
中科院分区：
文献类型：
--
作者：
Bukholm, G;Tannaes, T;Guldvog, I
背景资料：迄今为止，幽门螺杆菌感染后疾病表达的差异与宿主因素和细菌菌株间变异有关。在这项研究中，自发和生态介导的菌株内变异进行了检查。方法：对临床分离的4株H. pylori显示产生两种菌落形式。通过电子显微镜检查细菌形态。使用离子交换色谱法和SDS-PAGE检查细菌级分的蛋白质;使用薄层色谱法、脂质阴离子交换色谱法、硅胶柱色谱法、P-31-NMR、气相色谱法和质谱法检查脂质。在两种不同的系统中检测细菌的体外侵袭性和粘附性，并通过Western印迹分析测定尿素酶和VacA毒素。结果：H. pylori显示出产生两种菌落形式：在正常pH下，群体以L菌落为主。选择一个菌株进行进一步研究。细菌从L菌落保留VacA毒素和尿素酶，不侵入或粘附上皮细胞，并含有正常量的磷脂酰乙醇胺。在一个小的频率，自发的S集落形成。这些菌落中的细菌释放VacA和尿素酶，粘附并侵入上皮细胞，并含有增加量的溶血磷脂酰乙醇胺和磷脂酰丝氨酸。在向培养基（pH 6）中加入HCl后，几乎仅形成S菌落。结果表明，环境因素如HCl可改变细菌细胞壁，从而增强H. pylori的体外研究。一个类似的体内变化将对我们理解胃粘膜中HCl分泌和H之间的相互作用产生影响。幽门螺杆菌在消化性溃疡疾病的发展。
Background: Differences in expression of disease after infection with Heiicobacter pylori have so far been connected with host factors and bacterial interstrain variation. In this study, spontaneous and ecology-mediated intrastrain variation was examined. Methods: Four clinical isolates of H. pylori were shown to give rise to two colony forms. Bacterial morphology was examined by electron microscopy. Bacterial fractions were examined for proteins using ion exchange chromatography and SDS-PAGE; for lipids using thin-layer chromatography, lipid anion-exchange chromatography, column chromatography on silica gel, P-31-NMR, gas chromatography and mass spectrometry. Bacterial in vitro invasiveness and adhesiveness were examined in two different systems, and urease and VacA toxin were assayed by Western blot analysis. Results: H. pylori was shown to give rise to two colony forms: at normal pH the population was dominated by L colonies. One strain was chosen for further studies. Bacteria from L colonies retained VacA toxin and urease, did not invade or adhere to epithelial cells, and contained normal quantities of phosphatidylethanolamine. In a small frequency, spontaneous S colonies were formed. Bacteria from these colonies released VacA and urease, adhered to and invaded epithelial cells and contained increased amounts of lysophosphatidyl ethanolamine and phosphatidyl serine. After addition of HCl to the culture medium (pH 6), almost only S colonies were formed. The results demonstrate that environmental factors, such as HCl, can change the bacterial cell wall, and thereby enhance expression of virulence factors of H. pylori in vitro. A similar in vivo variation would have implications for our understanding of the interaction between HCl secretion in the gastric mucosa and H. pylori in the development of peptic ulcer disease.