Experimental asthma in guinea pigs sensitized with mites (Dermatophagoides farinae).
Experimental asthma in guinea pigs sensitized with mites (Dermatophagoides farinae).
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螨虫（Dermatophagoides farinae）致敏的豚鼠实验性哮喘。
DOI：
10.1159/000234982
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
T. Miyamoto
中科院分区：
文献类型：
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作者：
A. Ishii;K. Ito;Y. Ino;T. Miyamoto
用1、10、100或1000微克粉尘螨提取物蛋白与氢氧化铝凝胶混合免疫豚鼠，每28天3次。最后一次注射10天后，使用粉尘螨提取物进行支气管激发试验。1微克蛋白免疫组产生非常低水平的IgE-、IgG1-和IgG2抗体，并且在攻击时没有表现出哮喘反应。尽管10微克蛋白质免疫组的哮喘发病率高于其他组，但该组血清中的IgE抗体水平低于100微克或1000微克蛋白质免疫组。然而，10微克蛋白免疫组的IgE/IgG1、IgE/IgG2比值高于100微克和1000微克蛋白免疫组，而IgG1/IgG2抗体比值低于100微克和1000微克蛋白免疫组。
Guinea pigs were intraperitoneally immunized with 1, 10, 100 or 1,000 micrograms protein of an extract from mites (Dermatophagoides farinae) incorporated with aluminium hydroxide gel 3 times every 28 days. Ten days after the last injection, a bronchial provocation test was performed by the use of the mite extract. The group immunized with 1 microgram protein produced very low levels of IgE-, IgG1- and IgG2 antibodies, and did not show asthmatic response upon the challenge. Although the group immunized with 10 micrograms protein showed higher rates of asthmatic onset than other groups, the sera of this group contained lower levels of IgE antibodies than the group immunized with 100 or 1,000 micrograms protein. However, the sera of the group immunized with 10 micrograms protein showed higher ratios of IgE/IgG1, IgE/IgG2 and lower ratios of IgG1/IgG2 antibodies than the sera of the group immunized with 100 or 1,000 micrograms protein.