Rapid-scanning frequency-domain fluorometer with picosecond time resolution.
Rapid-scanning frequency-domain fluorometer with picosecond time resolution.
复制标题
具有皮秒时间分辨率的快速扫描频域荧光计。
DOI：
10.1364/ao.26.003526
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发表时间：
1987
期刊：
影响因子：
1.9
通讯作者：
G. Hieftje
中科院分区：
文献类型：
--
作者：
F. Bright;G. Hieftje
介绍了一种新的频域荧光计，它能够在10 MHz和1 GHz之间的频率范围内工作。该仪器采用锁模氩离子激光器作为82 MHz重复频率的激发源和一个廉价的腔外声光光调制器的二次频率的产生。该仪器是非常迅速的，可以获得，在~10秒，整个频谱需要确定激发态寿命。重要的是，这些频谱的特征在于，对于中等荧光信号（10-20微安检测器电流），信号-音调噪声比至少为20。使用这种新仪器，可以很容易地解决多个指数衰减，单指数衰减可以在优于20 ps的精度（标准偏差）确定。
A new frequency-domain fluorometer is described which is capable of operating over a frequency range between 10 MHz and 1 GHz. The instrument employs a mode-locked Ar-ion laser as an 82-MHz repetitionrate excitation source and an inexpensive extracavity acoustooptic light modulator for secondary-frequency generation. The instrument is very rapid and can acquire, in ~10 s, the entire frequency spectrum necessary to determine excited-state lifetimes. Importantly, these frequency spectra are characterized by signal-tonoise ratios of at least 20 for moderate fluorescence signals (10-20 microA detector current). With this new instrument, multiple exponential decays can be easily resolved, and single exponential decays can be determined at a precision (standard deviation) of better than 20 ps.