Expression of retinoic acid 4-hydroxylase (CYP26) during mouse and Xenopus laevis embryogenesis
Expression of retinoic acid 4-hydroxylase (CYP26) during mouse and Xenopus laevis embryogenesis
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DOI：
10.1016/s0925-4773(99)00016-7
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发表时间：
1999-04-01
影响因子：
2.6
通讯作者：
van der Saag, PT
中科院分区：
文献类型：
--
作者：
de Roos, K;Sonneveld, E;van der Saag, PT
视黄酸是脊椎动物胚胎发育过程中重要的信号分子，其合成和代谢受到严格调控。视黄酸（RA）4-羟化酶属于细胞色素P450家族CYP 26，是催化RA 4-羟基化的酶，从而调节RA稳态。在这里，我们描述了小鼠（mCYP 26）和非洲爪蟾（xCYP 26）同源物的时空表达模式。在小鼠中，在子宫隐窝、分化软骨周围、头部的几个区域、咽部区域、神经视网膜和躯干的几个区域中检测到表达。在非洲爪蟾中，北方印迹分析显示在MBT之前存在xCYP 26转录物，并且在原肠胚形成期间表达水平增加。整体原位杂交显示在原肠胚形成开始时出现的特定表达模式，在胚孔周围有一个环。到原肠胚形成中期，存在前部和后部表达域，其中每一个在发育后期变得更加复杂。非洲爪蟾和小鼠在表达模式上存在一些重要的相似性和差异。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Retinoids are important signal molecules during vertebrate embryonic development and their synthesis as well as catabolism should therefore be strictly regulated. The retinoic acid (RA) 4-hydroxylase, belonging to the cytochrome P450 family CYP26, is an enzyme catalyzing the 4-hydroxylation of RA, thereby regulating RA homeostasis. Here we describe the temporal and spatial expression patterns of mouse (mCYP26) and Xenopus laevis (xCYP26) homologues. In mouse, expression is detected in uterine crypt, around differentiating cartilage, several regions of the head, regions of the pharynx, the neural retina, and several regions of the trunk. In Xenopus, Northern blot analysis shows presence of xCYP26 transcripts before the MBT and an increased expression level during gastrulation. Whole-mount in situ hybridization shows a specific expression pattern arising at onset of gastrulation, with a ring around the blastopore. By mid gastrulation there is an anterior and a posterior expression domain, each of which gets more complex later in development. There are some important similarities and differences in expression pattern between Xenopus and mouse. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.