Systemic cytokine responses in patients with influenza-associated encephalopathy
Systemic cytokine responses in patients with influenza-associated encephalopathy
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DOI：
10.1086/377101
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发表时间：
2003-09-01
影响因子：
6.4
通讯作者：
Morishima, T
中科院分区：
文献类型：
--
作者：
Kawada, J;Kimura, H;Morishima, T
流感相关脑病是流感的一种严重神经系统并发症，在日本的报告更为频繁。我们研究了外周血白细胞中细胞因子基因的转录，并比较了流感合并脑病或热性惊厥患者和流感合并但无神经系统并发症的患者。定量聚合酶链反应（PCR）显示，与无神经系统并发症的脑病患者相比，脑病患者的白细胞介素(IL)-6、IL-10和肿瘤坏死因子- α基因的转录上调幅度更大。脑病患者血浆IL-6水平也较高，但差异不大。采用实时定量PCR对咽拭子中的病毒RNA进行定量。脑病、热性惊厥或无神经系统并发症患者的病毒载量相似。此外，病毒载量与细胞因子基因的转录或血浆细胞因子浓度无关。这些结果提示流感相关脑病可能是全身免疫反应的结果。
Influenza-associated encephalopathy, a severe neurologic complication of influenza, is being reported more frequently in Japan. We investigated the transcription of cytokine genes in peripheral blood leukocytes and compared patients with influenza and with encephalopathy or febrile convulsions and patients with influenza but without neurologic complications. A quantitative polymerase chain reaction (PCR) revealed that transcription of the interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha genes was up-regulated to a greater extent in patients with encephalopathy than in those without neurologic complications. Plasma IL-6 levels also were higher in patients with encephalopathy, although the difference was marginal. Viral RNA in throat swabs was quantified using a real-time quantitative PCR. The virus load was similar among patients with encephalopathy or febrile convulsions or without neurologic complications. Furthermore, virus load was not correlated with either the transcription of cytokine genes or plasma cytokine concentrations. These results suggest that influenza-associated encephalopathy might be a consequence of systemic immune responses.