Dedicated farnesyl diphosphate synthases circumvent isoprenoid‐derived growth‐defense tradeoffs in Zea mays
Dedicated farnesyl diphosphate synthases circumvent isoprenoid‐derived growth‐defense tradeoffs in Zea mays
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专用的法呢基二磷酸合酶规避了玉米中类异戊二烯衍生的生长和防御权衡
DOI：
10.1111/tpj.15941
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Akhtar, Tariq A.
中科院分区：
文献类型：
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作者：
Tang, Hoang V.;Berryman, David L.;Mendoza, Jorrel;Yactayo‐Chang, Jessica P.;Li, Qin‐Bao;Christensen, Shawn A.;Hunter, Charles T.;Best, Norman;Soubeyrand, Eric;Akhtar, Tariq A.
玉米（玉蜀黍）制造植物抗毒素，如倍半萜类zealexins，以对抗入侵的病原体。Zealexin由法呢基二磷酸以微克/克鲜重的量产生。由于法呢基二磷酸也是植物生长所必需的许多化合物的前体，因此出现了关于Z。mays产生高水平的zealexins，而不会对重要的植物系统产生负面影响。为了检查是否为zealexin合成制备了特定的法呢基二磷酸池，我们在Z中对三种法呢基二磷酸脱氢酶（FPS）中的每一种进行了CRISPR/Cas9敲除。maysand检查了对不同法呢基二磷酸衍生代谢物的影响。我们发现，FPS 3（GRMZM 2G 098569）产生了大部分用于zealexins的法呢基二磷酸，而FPS 1（GRMZM 2G 168681）产生了大部分用于重要呼吸辅因子泛醌的法呢基二磷酸。事实上，fps 1突变体有很强的发育表型，如降低身高和褪绿的发展。研究了fps基因家族在Z. maysenable它生产专用的FPS发育相关的泛醌生产（FPS 1）或防御相关的zealexin生产（FPS 3）。法呢基二磷酸的生产在生长和防御之间的这种分配可能有助于Z。可能会产生高水平的植物抗毒素，而不会对其生长产生负面影响。
Zea mays(maize) makes phytoalexins such as sesquiterpenoid zealexins, to combat invading pathogens. Zealexins are produced from farnesyl diphosphate in microgram per gram fresh weight quantities. As farnesyl diphosphate is also a precursor for many compounds essential for plant growth, the question arises as to howZ. maysproduces high levels of zealexins without negatively affecting vital plant systems. To examine if specific pools of farnesyl diphosphate are made for zealexin synthesis we made CRISPR/Cas9 knockouts of each of the three farnesyl diphosphate synthases (FPS) inZ. maysand examined the resultant impacts on different farnesyl diphosphate‐derived metabolites. We found that FPS3 (GRMZM2G098569) produced most of the farnesyl diphosphate for zealexins, while FPS1 (GRMZM2G168681) made most of the farnesyl diphosphate for the vital respiratory co‐factor ubiquinone. Indeed,fps1mutants had strong developmental phenotypes such as reduced stature and development of chlorosis. The replication and evolution of thefpsgene family inZ. maysenabled it to produce dedicated FPSs for developmentally related ubiquinone production (FPS1) or defense‐related zealexin production (FPS3). This partitioning of farnesyl diphosphate production between growth and defense could contribute to the ability ofZ. maysto produce high levels of phytoalexins without negatively impacting its growth.