Electrical coupling synchronises spinal motoneuron activity during swimming in hatchling Xenopus tadpoles
Electrical coupling synchronises spinal motoneuron activity during swimming in hatchling Xenopus tadpoles
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电耦合使非洲爪蟾蝌蚪幼体游泳时的脊髓运动神经元活动同步
DOI：
10.1113/jphysiol.2009.173468
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
K. Sillar
中科院分区：
文献类型：
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作者：
Hong;Wen;W. J. Heitler;K. Sillar
神经元之间的电耦合在非洲爪蟾胚胎的游泳节律发生器的作用进行了研究，使用药理学阻断缝隙连接。18β-甘草次酸（18β-GA）和甘珀酸（carbenoxolone）的一个显著作用是在游泳过程中增加整个脊髓腹根爆发的持续时间，它们已被证明可以阻断该制剂中的电耦合。左右协调、游泳频率和游泳事件的持续时间不受18β-GA浓度的影响，其显著增加爆发持续时间。然而，纵向耦合受到影响，使得18β-GA导致吻尾延迟和周期之间的显着相关性，这通常只存在于较老的幼虫动物中。脊髓运动神经元膜片钳记录测试是否间隙连接阻滞剂影响尖峰时间和/或发射模式的运动神经元在虚构的游泳。在存在18个β-GA的情况下，运动神经元在每个游泳周期中继续发射单个但更广泛的动作电位，并且它们的尖峰相对于前根爆发的时间变得更加可变。18β-GA对运动神经元的静息膜电位没有可检测的影响，但导致输入电阻显著增加，与缝隙连接阻断一致。这种效应并没有导致在游泳过程中增加发射，尽管事实上，多个尖峰可以发生在响应电流注入。在幼虫42期应用18β-GA对运动没有明显影响。结果表明，电耦合的主要功能，以同步活动的协同运动神经元在胚胎游泳，在运动系统发育的背景下进行了讨论。
The role of electrical coupling between neurons in the swimming rhythm generator of Xenopus embryos has been studied using pharmacological blockade of gap junctions. A conspicuous effect of 18β‐glycyrrhetinic acid (18β‐GA) and carbenoxolone, which have been shown to block electrical coupling in this preparation, was to increase the duration of ventral root bursts throughout the spinal cord during swimming. The left‐right coordination, the swimming frequency and the duration of swimming episodes were not affected by concentrations of 18β‐GA which significantly increased burst durations. However, the longitudinal coupling was affected such that 18β‐GA led to a significant correlation between rostrocaudal delays and cycle periods, which is usually only present in older larval animals. Patch clamp recordings from spinal motoneurons tested whether gap junction blockers affect the spike timing and/or firing pattern of motoneurons during fictive swimming. In the presence of 18β‐GA motoneurons continued to fire a single, but broader action potential in each cycle of swimming, and the timing of their spikes relative to the ventral root burst became more variable. 18β‐GA had no detectable effect on the resting membrane potential of motoneurons, but led to a significant increase in input resistance, consistent with the block of gap junctions. This effect did not result in increased firing during swimming, despite the fact that multiple spikes can occur in response to current injection. Applications of 18β‐GA at larval stage 42 had no discernible effect on locomotion. The results, which suggest that electrical coupling primarily functions to synchronize activity in synergistic motoneurons during embryo swimming, are discussed in the context of motor system development.
在体外大鼠腰脊髓中研究运动神经元电紧张耦合的出生后变化。
DOI：
10.1113/jphysiol.1991.sp018426
发表时间：
1991
期刊：
The Journal of physiology
影响因子：
--
作者：
Walton,KD;Navarrete,R
通讯作者：
Navarrete,R
血清素调节对水蛭学习至关重要的神经元之间的轴突耦合。
DOI：
10.1152/jn.00322.2005
发表时间：
2005
期刊：
Journal of neurophysiology.
影响因子：
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作者：
Moss,BrendaL;Fuller,AbbyD;Sahley,ChristieL;Burrell,BrianD
通讯作者：
Burrell,BrianD
间隙连接阻滞剂甘草次酸对胃肠道平滑肌细胞的影响。
DOI：
10.1152/ajpgi.00389.2004
发表时间：
2005
期刊：
American journal of physiology. Gastrointestinal and liver physiology.
影响因子：
--
作者：
Takeda,Yukari;Ward,SeanM;Sanders,KentonM;Koh,SangDon
通讯作者：
Koh,SangDon