African serum interference in the determination of chloroquine sensitivity in Plasmodium falciparum.
African serum interference in the determination of chloroquine sensitivity in Plasmodium falciparum.
复制标题
非洲血清干扰恶性疟原虫氯喹敏感性测定。
DOI：
10.1007/bf00926589
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Akood,MA
中科院分区：
文献类型：
--
作者：
Carlin,JM;VandeWaa,JA;Jensen,JB;Akood,MA
采用WHO微量技术程序和改良的48小时体外耐药性试验，对从苏丹中部村民分离的恶性疟原虫进行氯喹和甲氟喹敏感性试验。未发现耐药菌株。在WHO的程序中，寄生虫在患者血浆中培养，72%的分离株未能成熟到鞭毛期，但当感染的红细胞从患者血浆中洗出并在合并的非免疫血清中培养时，只有28.8%的分离株未能发育到鞭毛期。在随后的实验中，从P.使用感染恶性疟原虫的患者或来自未感染的“免疫”成人的样本来补充含有已知对氯喹敏感的寄生虫的标准体外试验平板。来自疟疾感染患者或免疫成人的血清在有或没有药物的情况下延缓了寄生虫的发育。这些体液因子和抗疟药的作用是相加的。在非洲分离株的药物敏感性试验中，建议用非免疫血清代替患者血浆。
Isolates ofPlasmodium falciparumfrom villagers in central Sudan were tested for chloroquine and mefloquine sensitivity using the WHO microtechnique procedure and a modified 48-h in vitro test for drug resistance. No drug-resistant strains were noted. In the WHO procedure, in which parasites were cultivated in the presence of the patient's plasma, 72% of the isolates failed to mature to the schizont stage, but when infected erythrocytes were washed free of the patient's plasma and cultivated in pooled nonimmune serum only 28.8% of the isolates failed to develop to the schizont stage. In subsequent experiments, sera fromP. falciparum-infected patients or from noninfected “immune” adults were used to supplement standard in vitro test plates which contained parasites of known chloroquine sensitivities. Sera from malaria-infected patients or from immune adults retarded parasite development in the presence or absence of drug. The effect of these humoral factors and the antimalarial drugs was additive. The replacement of the patient's plasma with nonimmune serum in drug sensitivity tests performed with African isolates is recommended.