Construction and Structure Determination of a Three-Dimensional DNA Crystal.
Construction and Structure Determination of a Three-Dimensional DNA Crystal.
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DOI：
10.1021/jacs.6b06508
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发表时间：
2016-08
影响因子：
15
通讯作者：
C. Simmons;Fei Zhang;J. Birktoft;Xiaodong Qi;Dongran Han;Yan Liu;R. Sha;Hatem O Abdallah;C. Hernandez;Yoel P. Ohayon;N. Seeman;Hao Yan
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文献类型：
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作者：
C. Simmons;Fei Zhang;J. Birktoft;Xiaodong Qi;Dongran Han;Yan Liu;R. Sha;Hatem O Abdallah;C. Hernandez;Yoel P. Ohayon;N. Seeman;Hao Yan
结构DNA纳米技术将分支DNA连接点与粘性末端结合起来，以创建自组装大分子结构。结构DNA纳米技术的关键目标之一是构建三维（3D）晶格。在这里，我们提出了一个新的DNA基序和一个战略，导致组装的三维晶格。我们已经确定了X射线晶体结构的两个相关的建设，以3.1毫米分辨率使用溴衍生的晶体。我们使用的基序采用了五个核苷酸的重复序列，通过一系列的两转DNA双链体编织。双链体被捆绑成一个分层结构，该结构由四臂接头的音乐会组织和支配;这些又组装成连续阵列，由序列特异性粘端凝聚力促进。这些DNA晶体的3D X射线结构有望设计新的结构基序，以创建具有原子空间分辨率的可编程3D DNA晶格。这两个阵列的不同之处在于在重复但统计学上无序的中心链中使用了四个或六个重复的五个核苷酸单元。此外，我们报告了一个二维菱形阵列形成类似的组件。
Structural DNA nanotechnology combines branched DNA junctions with sticky-ended cohesion to create self-assembling macromolecular architectures. One of the key goals of structural DNA nanotechnology is to construct three-dimensional (3D) crystalline lattices. Here we present a new DNA motif and a strategy that has led to the assembly of a 3D lattice. We have determined the X-ray crystal structures of two related constructs to 3.1 Å resolution using bromine-derivatized crystals. The motif we used employs a five-nucleotide repeating sequence that weaves through a series of two-turn DNA duplexes. The duplexes are tied into a layered structure that is organized and dictated by a concert of four-arm junctions; these in turn assemble into continuous arrays facilitated by sequence-specific sticky-ended cohesion. The 3D X-ray structure of these DNA crystals holds promise for the design of new structural motifs to create programmable 3D DNA lattices with atomic spatial resolution. The two arrays differ by the use of four or six repeats of the five-nucleotide units in the repeating but statistically disordered central strand. In addition, we report a 2D rhombuslike array formed from similar components.