Dissociation between adipose tissue expression and serum levels of adiponectin during and after diet-induced weight loss in obese subjects with and without the metabolic syndrome
Dissociation between adipose tissue expression and serum levels of adiponectin during and after diet-induced weight loss in obese subjects with and without the metabolic syndrome
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DOI：
10.1016/j.metabol.2007.03.010
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发表时间：
2007-08-01
影响因子：
9.8
通讯作者：
Carlsson, Lena M. S.
中科院分区：
文献类型：
--
作者：
Behre, Carl Johan;Gummesson, Anders;Carlsson, Lena M. S.
该研究旨在检查在基线时代谢异常受试者（MetS+）是否具有比非代谢异常肥胖受试者（MetS-）更低的脂联素基因表达和更低的循环脂联素水平，如果在减肥期间代谢异常组中脂联素表达和脂联素浓度升高更多，并且如果V-SNARE Vti I a（囊泡转运可溶性NSF附着蛋白受体vps 10 p尾相互作用I a）表达在体重减轻期间增加，作为增加脂联素分泌的机制。21名肥胖MetS+和19名肥胖MetS-受试者接受了16周的极低能量饮食，随后进行了2周的再喂养。腹部皮下脂肪组织活检和血液样本之前，期间，节食后的DNA微阵列，逆转录聚合酶链反应，和生化分析。血清脂联素也在性别和年龄匹配的健康，非肥胖的参考组进行了评估。MetS+组体重下降26.3 +/- 9.8 kg，MetS-组体重下降28.2 +/- 8.4 kg，同时胰岛素、血红蛋白A（1c）和甘油三酯下降，在MetS+组中更为明显。最初，MetS+受试者的血清脂联素较低，但差异在第8周消失，在整个研究期间，两组的血清脂联素持续增加，达到高于参考组的水平。脂联素和v-SNARE Vti I a的表达在组间或随时间推移没有差异。总之，患有代谢综合征的肥胖患者的循环脂联素水平低于无代谢综合征的患者。体重减轻增加血清脂联素水平，但脂联素基因表达没有平行增加。脂联素水平的调节机制值得进一步研究。(c)2007年爱思唯尔公司All rights reserved.
The study aimed to examine if dysmetabolic subjects (MetS+) have lower adiponectin gene expression and lower circulating adiponectin levels than non-dysmetabolic obese subjects (MetS-) at baseline, if adiponectin expression and adiponectin concentration rise more in the dysmetabolic group during weight loss, and if v-SNARE Vti I a (vesicle transport soluble NSF attachment protein receptor vps 10p tail interacting I a) expression increases during the weight loss, as a mechanism for increased adiponectin secretion. Twenty-one obese MetS+ and 19 obese MetS- subjects underwent a very low-energy diet for 16 weeks followed by 2 weeks of refeeding. Abdominal subcutaneous adipose tissue biopsies and blood samples were taken before, during, and after dieting for DNA microarray, reverse transcriptase-polymerase chain reaction, and biochemical analyses. Serum adiponectin was also assessed in a sex- and age-matched healthy, nonobese reference group. Weight decreased by 26.3 +/- 9.8 kg in the MetS+ group and 28.2 +/- 8.4 kg in the MetS- group with concomitant reductions in insulin, hemoglobin A(1c), and triglycerides that were more pronounced in the MetS+ group. Initially, the MetS+ subjects had lower serum adiponectin, but the differences disappeared at week 8, with a continuous increase in serum adiponectin throughout the study in both groups to a level that was higher than in the reference group. The expression of adiponectin and v-SNARE Vti I a did not differ between the groups or over time. In conclusion, obese subjects with the metabolic syndrome had lower circulating adiponectin than subjects without the syndrome. Weight loss increased serurn levels of adiponectin without a parallel increase in adiponectin gene expression. The mechanisms involved in the regulation of adiponectin levels merits further investigation. (c) 2007 Elsevier Inc. All rights reserved.