Gene expression during preimplantation mouse development.
Gene expression during preimplantation mouse development.
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DOI：
10.1101/gad.6.7.1190
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发表时间：
1992-07
影响因子：
10.5
通讯作者：
J. Rothstein;Dabney K. Johnson;J. Deloia;J. Skowroński;D. Solter;B. Knowles
中科院分区：
文献类型：
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作者：
J. Rothstein;Dabney K. Johnson;J. Deloia;J. Skowroński;D. Solter;B. Knowles
为了开发用于鉴定和分离在早期哺乳动物胚胎中表达的基因的资源，从未受精卵、两细胞、八细胞和囊胚期小鼠胚胎构建了大的和代表性的cDNA文库。使用这些库，我们现在报告的第一阶段，在其中细胞因子白细胞介素（IL）-6，IL-1 β和干扰素（IFN）-γ在发育中的胚胎和IL-7的转录本的存在下，在未受精卵转录。在这些阶段未检测到IL-1 α、-2、-3、-4或-5的转录物。为了鉴定在胚胎基因组激活时表达的新基因，从双细胞文库中减去卵和八细胞阶段特异性cDNA文库，产生富集在双细胞阶段表达的转录物的专门cDNA文库。对这些主要在胚胎发生的两细胞阶段表达的cDNA进行序列和Southern印迹分析，发现它们来自新基因，从而为研究早期哺乳动物胚胎中的基因表达提供了第一个分子工具。
To develop a resource for the identification and isolation of genes expressed in the early mammalian embryo, large and representative cDNA libraries were constructed from unfertilized eggs, and two-cell, eight-cell, and blastocyst-stage mouse embryos. Using these libraries, we now report the first stages at which the cytokines interleukin (IL)-6, IL-1 beta, and interferon (IFN)-gamma are transcribed in the developing embryo and the presence of IL-7 transcripts in the unfertilized egg. Transcripts for IL-1 alpha, -2, -3, -4, or -5 were not detected at these stages. To identify novel genes expressed on activation of the embryonic genome, the egg and eight-cell stage-specific cDNA libraries were subtracted from the two-cell library, yielding a specialized cDNA library enriched for transcripts expressed at the two-cell stage. Sequence and Southern blot analysis of several of these cDNAs expressed predominantly at the two-cell stage of embryogenesis revealed them to be from novel genes, thereby providing the first molecular tools with which to approach the study of gene expression in the early mammalian embryo.