ExoCarta: A compendium of exosomal proteins and RNA
ExoCarta: A compendium of exosomal proteins and RNA
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DOI：
10.1002/pmic.200900351
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发表时间：
2009-11-01
期刊：
影响因子：
3.4
通讯作者：
Simpson, Richard J.
中科院分区：
文献类型：
--
作者：
Mathivanan, Suresh;Simpson, Richard J.
外泌体是由大多数细胞类型分泌的膜微囊泡（40-100 nm），可以以多种方式分离，而表征它们在很大程度上基于电子显微镜，最重要的是，鉴定外泌体标记蛋白。研究人员依靠对某些外泌体标记蛋白（包括阿利克斯、CD 9和CD 63）的鉴定来确认其制剂中外泌体的存在。迄今为止，在大多数研究的外泌体中已经鉴定出一组进化保守的蛋白质分子。然而，由于组织/细胞类型特异性蛋白质被掺入外泌体中的复杂性，这些所谓的外泌体标志物中的一些并不总是存在于所有外泌体中。外泌体中组织/细胞类型特异性蛋白的存在允许研究人员使用免疫亲和捕获方法分离它们。外泌体蛋白质组的概要将有助于研究人员识别在各种外泌体中更常见的蛋白质（外泌体标记物）以及对某些组织/细胞类型来源的外泌体特异的蛋白质。在这里，我们描述了ExoCarta，一个在外来体中鉴定的蛋白质和RNA分子的纲要。ExoCarta是第一个此类网站，科学界可通过网络（http：//www.example.com）免费获取该资源。exocarta.ludwig.edu.au我们相信，这种社区资源将是非常重要的生物学的任何未来外来体分析。
Exosomes, membrane microvesicles (40-100 nm) secreted by most cell types, can be isolated in several ways while characterizing them is heavily based on electron microscopy and, most importantly, the identification of exosome marker proteins. Researchers rely on the identification of certain exosomal marker proteins including Alix, CD9 and CD63 to confirm the presence of exosomes in their preparations. An evolutionary-conserved set of protein molecules have been identified in most exosomes studied to date. However, with the complexity of tissue/cell type-specific proteins being incorporated in the exosomes, some of these so-called exosomal markers are not always present in all the exosomes. The presence of tissue/cell type-specific proteins in exosomes allows researchers to isolate them using immunoaffinity capture methods. A compendium for exosomal proteomes will aid researchers in identifying proteins that were more commonly found in various exosomes (exosome markers) and those that are specific to certain tissue/cell type-derived exosomes. Here, we describe ExoCarta, a compendium for proteins and RNA molecules identified in exosomes. ExoCarta is first of its kind and the resource is freely available to the scientific community through the web (http://exocarta.ludwig.edu.au). We believe that this community resource will be of great biological importance for any future exosome analyses.