Modulating the synthetase activity of penicillin G acylase in organic media by addition of N-methylimidazole: using vinyl acetate as activated acyl donor.
Modulating the synthetase activity of penicillin G acylase in organic media by addition of N-methylimidazole: using vinyl acetate as activated acyl donor.
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DOI：
10.1016/j.jbiotec.2011.03.009
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发表时间：
2011-05
影响因子：
4.1
通讯作者：
Bokai Liu;Qi Wu;De-shui Lv;Xianfu Lin
中科院分区：
文献类型：
--
作者：
Bokai Liu;Qi Wu;De-shui Lv;Xianfu Lin
本文报道了有机小分子对酶活性的调节作用。青霉素G酰化酶（PGA）的酯化活性在添加10%的N-甲基咪唑后提高了70倍以上。已经设计了一些对照实验来证明PGA的催化特异性。优化了添加剂的结构和用量，提高了产品收率。通过FTIR和Autodock模拟研究了N-甲基咪唑对PGA构象的影响。使用七种底物来评估结构对PGA催化的酯交换的影响。在10%的N-甲基咪唑存在下，PGA对含苯基的底物表现出特异性识别，产率为56%~ 84%.
This paper reported the modulation of enzyme activity by organic small molecule. The esterification activity of Penicillin G acylase (PGA) was improved more than 70-fold by the addition of 10%N-methylimidazole. Some control experiments have been designed to demonstrate the catalytic specificity of PGA. The structure and the amount of additive were optimized to improve the product yield. The influence ofN-methylimidazole on the PGA conformation was investigated by FTIR and autodock simulation. Seven substrates were used to evaluate the effect of structure on the PGA-catalyzed transesterification. A series of products were successfully synthesized with the yield ranged from 56% to 84% and PGA showed specific recognition on the substrate with phenyl group in the presence of 10%N-methylimidazole.