Spermatogenesis-related ring finger gene ZNF230 promoter: identification and functional analysis
Spermatogenesis-related ring finger gene ZNF230 promoter: identification and functional analysis
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DOI：
10.1007/s11033-008-9296-2
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发表时间：
2009-05
影响因子：
2.8
通讯作者：
Wenming Xu;Si-zhong Zhang;W. Qiu;Guo-ping He;Yunqiang Liu;Yan Sun;Yongxin Ma;Jingtao Dong;Wei Zhang
中科院分区：
文献类型：
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作者：
Wenming Xu;Si-zhong Zhang;W. Qiu;Guo-ping He;Yunqiang Liu;Yan Sun;Yongxin Ma;Jingtao Dong;Wei Zhang
TheZNF230gene is a recently cloned gene which is transcribed only in fertile male testes and may be related to human spermatogenesis. To characterize the multiple stage-specific transcription elements necessary forZNF230expression, we cloned ZNF230 promoter and constructed chimeric luciferase reporter Plasmids. Overexpression and site-directed mutation test were used to characterize thecis-element. The results showedZNF230gene promoter to be GC rich and not contain a TATA box. Deletion analysis of the 5′-flanking region ofZNF230in HEK293 cells indicated that the sequence encompassing from nt −131 to +152 has a basal transcriptional activity. Site-directed mutation test and mithramycin A treatment demonstrated that theZNF230promoter contained a functional Sp1 site. Overexpression of theSox5protein activated the promoter activity. A 312-bp fragment surrounding the transcription start site exhibits a characteristic CpG island which overlaps with the promoter region. We also provided evidence that both the human and mouseznf230promoter consist of Sp1 binding site and GC-rich sequences, suggesting Sp1 is required for the transcription of human and mouseZNF230genes. In conclusion, these findings suggest thatZNF230is tightly controlled at transcriptional level and a common mechanism controls the basal transcription ofZNF230gene.
