Bipolar localization of a chromosome partition protein in Bacillus subtilis
Bipolar localization of a chromosome partition protein in Bacillus subtilis
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DOI：
10.1073/pnas.94.9.4721
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发表时间：
1997-04-29
影响因子：
11.1
通讯作者：
Grossman, AD
中科院分区：
文献类型：
--
作者：
Lin, DCH;Levin, PA;Grossman, AD
我们已经确定了亚细胞定位的染色体分配蛋白Spo 0 J的枯草芽孢杆菌的免疫荧光显微镜和可视化的Spo 0 J-GFP融合蛋白的荧光。Spo 0 J与细胞极附近的类核区域相关，无论是在对称分裂的生长细胞中还是在不对称分裂的产孢细胞中。另外的实验表明Spo 0 J被结合到染色体的起始近端三分之一的位点上。这些结果表明，复制染色体在分裂周期中以特定的方式定向，Spo 0 J结合区域朝向细胞两极定位。在细胞周期的不同阶段的细胞特征的实验表明，染色体的方向是建立在细胞分裂开始之前。我们的研究结果表明，有一种机制，定向的染色体和染色体分配蛋白Spo 0 J可能是一个细菌有丝分裂样装置的一部分。
We have determined the subcellular localization of the chromosome partition protein Spo0J of Bacillus subtilis by immunofluorescence microscopy and visualizing fluorescence of a Spo0J-GFP fusion protein. Spo0J was associated with a region of the nucleoid proximal to the cell pole, both in growing cells dividing symmetrically and in sporulating cells dividing asymmetrically. Additional experiments indicated that Spo0J was bound to sites in the origin-proximal third of the chromosome. These results show that the replicating chromosomes are oriented in a specific manner during the division cycle, with the Spo0J binding region positioned toward the cell poles. Experiments characterizing cells at different stages of the cell cycle showed that chromosome orientation is established prior to the initiation of cell division. Our results indicate that there is a mechanism for orienting the chromosomes and that the chromosome partition protein Spo0J might be part of a bacterial mitotic-like apparatus.