Isolation and transmission of the Lyme disease spirochete from the southeastern United States.
Isolation and transmission of the Lyme disease spirochete from the southeastern United States.
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美国东南部莱姆病螺旋体的分离和传播。
DOI：
10.1073/pnas.90.15.7371
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发表时间：
1993
影响因子：
11.1
通讯作者：
Lane,RS
中科院分区：
文献类型：
--
作者：
OliverJr,JH;ChandlerJr,FW;Luttrell,MP;James,AM;Stallknecht,DE;McGuire,BS;Hutcheson,HJ;Cummins,GA;Lane,RS
本文报道从美国东南部分离到莱姆病螺旋体（伯氏疏螺旋体）。1991年7月和9月，从格鲁吉亚的萨佩洛岛回收了三个分离株，两个来自棉鼠（Peromyscus gossypinus），一个来自黑腿蜱（Ixodes scapularis）。通过使用一组五种单克隆抗体的间接荧光抗体测定、全细胞裂解物的十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS/PAGE）和使用B中发现的三种DNA靶序列的引物的聚合酶链反应（PCR）测定来表征螺旋体。burgdorferi参考菌株B-31。传播实验表明，三个格鲁吉亚分离株可以感染实验接种的仓鼠和小鼠。到目前为止，已经尝试了其中一个分离株的蜱传播; scapularis将分离物SI-1从仓鼠传播到小鼠，但孤星蜱Amblyomma americanum没有。
The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not.