In situ identification of intracellular bacteria related to Paenibacillus spp. in the mycelium of the ectomycorrhizal fungus Laccatia bicolor S238N
In situ identification of intracellular bacteria related to Paenibacillus spp. in the mycelium of the ectomycorrhizal fungus Laccatia bicolor S238N
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DOI：
10.1128/aem.69.7.4243-4248.2003
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Frey-Mett, P
中科院分区：
文献类型：
--
作者：
Bertaux, J;Schmid, M;Frey-Mett, P
过去 15 年里，在外生菌根真菌 Laccaria bicolor S238N 的发酵罐培养物中反复观察到细菌增殖，表明这种真菌的收集培养物中存在隐秘细菌。在本研究中，通过荧光原位杂交结合共聚焦激光扫描显微镜在双色乳杆菌 S238N 的几个收集传代培养物中检测细胞内细菌。它们体积小（直径为0.5μm）、稀有且在菌丝体中分布不均匀，被鉴定为类芽孢杆菌属（Paenibacillus spp）。通过使用最初为从 L. bicolor S238N 发酵罐培养物中分离的细菌设计的 16S rRNA 定向寡核苷酸探针。
Bacterial proliferations have recurrently been observed for the past 15 years in fermentor cultures of the ectomycorrhizal fungus Laccaria bicolor S238N, suggesting the presence of cryptic bacteria in the collection culture of this fungus. In this study, intracellular bacteria were detected by fluorescence in situ hybridization in combination with confocal laser scanning microscopy in several collection subcultures of L. bicolor S238N. They were small (0.5 mum in diameter), rare, and heterogeneously distributed in the mycelium and were identified as Paenibacillus spp. by using a 16S rRNA-directed oligonucleotide probe initially designed for bacteria isolated from a fermentor culture of L. bicolor S238N.