Engineering a Novel Modular Adenoviral mRNA Delivery Platform Based on Tag/Catcher Bioconjugation.
Engineering a Novel Modular Adenoviral mRNA Delivery Platform Based on Tag/Catcher Bioconjugation.
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构建一种基于Tag/Catcher生物偶联的新型模块化腺病毒mRNA传递平台。
DOI：
10.3390/v15112277
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发表时间：
2023-11-20
期刊：
影响因子：
--
通讯作者：
Curiel DT
中科院分区：
文献类型：
--
作者：
Geng K;Rice-Boucher PJ;Kashentseva EA;Dmitriev IP;Lu ZH;Goedegebuure SP;Gillanders WE;Curiel DT
mRNA vaccines have attracted widespread research attention with clear advantages in terms of molecular flexibility, rapid development, and potential for personalization. However, current mRNA vaccine platforms have not been optimized for induction of CD4/CD8 T cell responses. In addition, the mucosal administration of mRNA based on lipid nanoparticle technology faces challenges in clinical translation. In contrast, adenovirus-based vaccines induce strong T cell responses and have been approved for intranasal delivery. To leverage the inherent strengths of both the mRNA and adenovirus platforms, we developed a novel modular adenoviral mRNA delivery platform based on Tag/Catcher bioconjugation. Specifically, we engineered adenoviral vectors integrating Tag/Catcher proteins at specific locales on the Ad capsid proteins, allowing us to anchor mRNA to the surface of engineered Ad viruses. In proof-of-concept studies, the Ad-mRNA platform successfully mediated mRNA delivery and could be optimized via the highly flexible modular design of both the Ad-mRNA and protein bioconjugation systems.
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DOI：
10.1056/nejmoa2034577
发表时间：
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期刊：
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影响因子：
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期刊：
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影响因子：
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