Rhodoligotrophos defluvii sp. nov., isolated from activated sludge
Rhodoligotrophos defluvii sp. nov., isolated from activated sludge
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河红寡养菌 sp.
DOI：
10.1099/ijsem.0.003691
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发表时间：
2019
影响因子：
2.8
通讯作者：
Li Qiang
中科院分区：
文献类型：
--
作者：
Liu Yu-Ling;Meng Dong;Li Rui-Rui;Gu Peng-Fei;Fan Xiang-Yu;Huang Zhao-Song;Du Zong-Jun;Li Qiang
从滨州（中国山东省）的废水处理设施获得的环氧丙烷皂化废水活性污泥中分离到革兰氏染色阴性、不形成孢子、不运动、短杆状且好氧的细菌菌株（命名为lm 1 T）。菌株lml T在15 ° C和45 °C之间生长（最佳，40 °C）。生长的pH范围为pH 4.0-10.0（最适生长pH 8.0）。菌株lm 1 T生长的NaCl浓度范围为0- 4.0%（w/v），最适生长浓度为1.0- 2.0%（w/v）。 基于16 S rRNA基因序列的系统发育分析表明，菌株lm 1 T属于红寡养虫属，与附属红寡养虫JCM 16873 T（16 S rRNA基因序列相似性为96.7%）和金胜红寡养虫CCTCC AB 2013083 T（96.2%）的亲缘关系较近。 菌株lm 1 TandR. JCM 16873 T的分离率分别为73.4%和14.3%。 菌株lm 1 T的主要脂肪酸为C19：0 cyclo ω 8 c、C18：1ω 7 c和C16：0。 菌株lm 1 T含有Q-10作为主要的呼吸醌。极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、二磷脂酰甘油、3种未鉴别氨基脂质、1种未鉴别糖脂和5种未知脂质组成。基因组DNA的G+C含量为64.4mol%。根据多相分类学数据，菌株lm 1 T可被分类为Rhodoligotrophos属的一个新种，命名为Rhodoligotrophos defluviisp.十一是建议。模式菌株为lm 1 T（=CCTCC AB 2019071 T =KCTC 72156 T）。
A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1T) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1Tgrew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0–10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1Twas 0–4.0 % (w/v), with optimum growth at 1.0–2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1Tbelonged to the genusRhodoligotrophosand was closely related toRhodoligotrophos appendiciferJCM 16873T(96.7 % 16S rRNA gene sequence similarity) andRhodoligotrophos jinshengiiCCTCC AB2013083T(96.2 %). The average nucleotide identity value and the digital DNA–DNA hybridization value between strain lm1TandR. appendiciferJCM 16873Twere 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1Twere C19 : 0cyclo ω8c, C18 : 1ω7cand C16 : 0. Strain lm1Tcontained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1Tcould be classified as a representative of a novel species of the genusRhodoligotrophos, for which the nameRhodoligotrophos defluviisp. nov. is proposed. The type strain is lm1T(=CCTCC AB2019071T=KCTC 72156T).