The critical stage for inducing oviparity and embryonic diapause in parthenogenetic Artemia (Crustacea: Anostraca): an experimental study
The critical stage for inducing oviparity and embryonic diapause in parthenogenetic Artemia (Crustacea: Anostraca): an experimental study
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孤雌生殖卤虫（甲壳纲：Anostraca）诱导卵生和胚胎滞育的关键阶段：一项实验研究
DOI：
10.1007/s00343-019-8322-7
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发表时间：
2019-09
影响因子：
1.6
通讯作者：
Sun Shichun
中科院分区：
文献类型：
--
作者：
Wang Zhichao;Asem Alireza;Okazaki Robert K.;Sun Shichun
卤虫有两种繁殖方式。1)。卵生的，产生滞育胚胎的；2)。卵生，产生自由游动的无节幼体。以往的研究表明，存在一个决定生殖方式的关键阶段。物理化学因素，如光周期、温度和盐度，被认为在这一关键阶段不可逆转地影响卵母细胞的繁殖模式。本研究利用对光周期和温度敏感的孤雌生殖卤虫克隆，将卤虫母体在卵生(18h L：6h D，27℃)和卵生(6h L：18h D，19℃)培养条件之间进行双向转移。在主要实验中(卤虫分6个不同的阶段，包括幼虫II到成虫II)，当幼虫II和III进行转移时，第一代的繁殖模式被转换，而当III之后的雌性转移时，第一代的繁殖模式不被转换。补充实验进一步表明，在“后IV期早期”转移雌性时，第一代的繁殖模式可以改变，特征是发育中的卵囊到达第三腹节中部，腹棘，一些卵母细胞生长得比其他卵更大。在这两个实验中，第二代幼虫的繁殖模式都受到了显著的影响。这些结果表明，诱导卵子繁殖和胚胎滞育的关键时期是卵母细胞的前期发育阶段，或者是第一代后代的母体“后第四代幼虫早期”。在这个阶段，两个目的卵母细胞的差异基因表达模式可能是由卵母细胞接收的令牌刺激信号触发的。
The brine shrimp Artemia exhibits two reproductive modes. 1). oviparity, producing diapause embryos; an. 2). ovoviviparity, producing free-swimming nauplii. Previous studies have suggested the existence of a critical stage that determines the reproductive mode. Physicochemical factors, such as photoperiod, temperature, and salinity, have been suggested to irreversibly affect the reproductive mode of oocytes during this critical stage. In this study, experiments were carried out using a photoperiod and temperature-sensitive parthenogenetic Artemia clone where maternal Artemia were shifted bidirectionally between ovoviviparity (18 h L:6 h D, 27°C) and oviparity (6 h L:18 h D, 19°C) culture conditions. In the main experiment (Artemia shifted at six different stages including the post-larva II to adult II), the reproductive mode of first brood was converted when shifting was performed on post-larva II and III but was not converted when females were shifted after post-larva III. A supplementary experiment further revealed that the reproductive mode of first brood could be altered when shifting females at an “early phase of post-larva IV”, characterized by a developing ovisac reaching the middle of the third abdominal segment, ventral spines, and some oocytes growing larger than the others. In both experiments, reproductive modes of the second brood were significantly affected when the shifting was performed on post-larva IV. These results suggest that the critical stage for inducing oviparity and embryonic diapause is at the previtellogenic stage of oocytes, or at maternal “early phase of post-larva IV” for the first-brood offspring. During this stage, differential gene expression patterns of the two destined oocytes may be triggered by the token stimuli signals received by the oocytes.
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影响因子：
2.6
作者：
G. Triantaphyllidis;Kiriaki Poulopoulou;T. Abatzopoulos;C. Perez;P. Sorgeloos
通讯作者：
G. Triantaphyllidis;Kiriaki Poulopoulou;T. Abatzopoulos;C. Perez;P. Sorgeloos
影响因子：
3.8
作者：
L. Zhang;H. Lefcort
通讯作者：
L. Zhang;H. Lefcort
影响因子：
2.6
作者：
Brendonck, L
通讯作者：
Brendonck, L
影响因子：
2.3
作者：
T. Wagner;E. Villavaso;J. Willers
通讯作者：
T. Wagner;E. Villavaso;J. Willers
DOI：
10.1007/978-1-4020-5680-2_1
发表时间：
2007
期刊：
--
影响因子：
--
作者：
V. Alekseev;O. Ravera;B. Stasio
通讯作者：
V. Alekseev;O. Ravera;B. Stasio