Chronic intake of high-dose of blueberry leaf extract does not augment the harmful effects of ethanol in rats
Chronic intake of high-dose of blueberry leaf extract does not augment the harmful effects of ethanol in rats
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DOI：
10.7717/peerj.6989
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发表时间：
2019-06-07
期刊：
影响因子：
2.7
通讯作者：
Yamasaki, Masao
中科院分区：
文献类型：
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作者：
Yamasaki, Kaede;Sugamoto, Kazuhiro;Yamasaki, Masao
过量饮酒是肝脏疾病的危险因素。酒精代谢的增强可能是一种有效的策略，以防止这些不利影响，因为它促进清除乙醇和乙醛从血清中。富含多酚的产品已被证明可以防止酒精相关的肝损伤。蓝莓叶因富含多酚类物质如原花青素和绿原酸而备受关注。在这项研究中，我们研究了高剂量的蓝莓叶提取物（BLEx）对酒精代谢的影响，在慢性摄入乙醇。7周龄SD大鼠随机分为4组：普通流质饮食组（NLD）、普通流质饮食+ BLEx组（NLD + BLEx）、酒精流质饮食组（ALD）和酒精流质饮食+ BLEx组（ALD + BLEx）。然后，给大鼠喂食实验饲料5周，在喂养期结束时，测量体重、摄食量、肝脏重量、肝损伤指数、酒精代谢相关酶和抗氧化酶的表达和活性、羰基蛋白、甘油三酯（TG）和总胆固醇（T-Chol）水平。体重和食物摄入量下降，而肝醛脱氢酶（ALDH）活性，肝微粒体细胞色素P450 2 E1（CYP 2 E1）蛋白和mRNA表达，血红素加氧酶1（HO-1）mRNA表达上调乙醇摄入量。然而，饮食BLEx并没有影响任何这些乙醇相关的变化。乙醇和BLEx并没有改变肝损伤指数、其他酒精代谢相关酶的表达和活性、肝羰基蛋白、TG和T-Chol水平。因此，慢性BLEx摄入不会改善乙醇的有害影响。
Excessive alcohol consumption is a risk factor for liver diseases. Enhancement of alcohol metabolism could be an effective strategy to prevent these adverse effects since it promotes the clearance of ethanol and acetaldehyde from the serum. Polyphenol-rich products have shown to protect against alcohol-related liver damage. Blueberry leaves have attracted attention as they are rich polyphenols such as proantocyanidins and chlorogenic acid. In this study, we investigated the effects of a high dose of blueberry leaf extract (BLEx) on alcohol metabolism during chronic intake of ethanol. Seven-week old Sprague-Dawley (SD) rats were divided into four groups: normal liquid diet group (NLD), normal liquid diet + BLEx group (NLD + BLEx), alcohol liquid diet group (ALD), and alcohol liquid diet + BLEx (ALD + BLEx). Then, rats were fed experimental diet for 5 weeks and at the end of feeding period, body weight, food intake, liver weight, indices of liver injury, expression and activity of alcohol metabolism-related and anti-oxidative enzymes, and levels of carbonyl protein, triglyceride (TG), and total cholesterol (T-Chol) were measured. Body weight and food intake decreased, whereas liver aldehyde dehydrogenase (ALDH) activity, liver microsomal cytochrome P450 2E1 (CYP2E1) protein and mRNA expression, and heme oxygenase 1 (HO-1) mRNA expression were upregulated by ethanol intake. Dietary BLEx, however, did not affect any of these ethanol-related changes. Indices of liver injury, expression and activity of other alcohol metabolism-related enzymes, liver carbonyl protein, TG, and T-Chol levels were not altered by ethanol and BLEx. Thus, chronic BLEx intake does not ameliorate the harmful effects of ethanol.