Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.
Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.
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DOI：
10.1084/jem.181.6.2213
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发表时间：
1995-06-01
期刊：
影响因子：
--
通讯作者：
Francke U
中科院分区：
文献类型：
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作者：
Bowen MA;Patel DD;Li X;Modrell B;Malacko AR;Wang WC;Marquardt H;Neubauer M;Pesando JM;Francke U
抗体阻断研究已经证实了CD 6在胸腺细胞-胸腺上皮（TE）细胞粘附中的作用。在这里，我们报告说，COS细胞表达的CD 6介导粘附到TE细胞，这种相互作用是专门阻断与抗CD 6单克隆抗体（mAb）或与单克隆抗体（J 4 -81），识别TE细胞抗原。我们分离并表达了编码该抗原的cDNA克隆，并显示用该cDNA转染的COS细胞结合CD 6免疫球蛋白融合蛋白（CD 6-Rg）。这种抗原，我们命名为ALCAM（活化的白细胞细胞粘附分子），因为它的表达活化的白细胞，似乎是人类同源的鸡神经粘附分子BEN/SC-1/DM-GRASP。用荧光原位杂交技术将该基因定位于人染色体3q13.1-q13.2。我们制备了ALCAM-Rg融合蛋白，并显示其与表达CD 6的COS细胞转染子结合，证明ALCAM是CD 6配体。ALCAM也由活化的白细胞表达，并且ALCAM和CD 6都在脑中表达的观察结果表明，ALCAM-CD 6相互作用可能在T和B细胞与活化的白细胞的结合中以及在神经系统细胞之间的相互作用中起作用。
Antibody-blocking studies have demonstrated the role of CD6 in thymocyte-thymic epithelial (TE) cell adhesion. Here we report that CD6 expressed by COS cells mediates adhesion to TE cells and that this interaction is specifically blocked with an anti-CD6 monoclonal antibody (mAb) or with a mAb (J4-81) that recognized a TE cell antigen. We isolated and expressed a cDNA clone encoding this antigen and show that COS cells transfected with this cDNA bind a CD6 immunoglobulin fusion protein (CD6-Rg). This antigen, which we named ALCAM (activated leukocyte-cell adhesion molecule) because of its expression on activated leukocytes, appears to be the human homologue of the chicken neural adhesion molecule BEN/SC-1/DM-GRASP. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. We prepared an ALCAM-Rg fusion protein and showed that it binds to COS cell transfectants expressing CD6, demonstrating that ALCAM is a CD6 ligand. The observations that ALCAM is also expressed by activated leukocytes and that both ALCAM and CD6 are expressed in the brain suggest that ALCAM-CD6 interactions may play a role in the binding of T and B cells to activated leukocytes, as well as in interactions between cells of the nervous system.