Genetic Diversity and Geographical Differentiation of Desmodium triflorum (L.) DC. in South China Revealed by AFLP Markers

Genetic Diversity and Geographical Differentiation of Desmodium triflorum (L.) DC. in South China Revealed by AFLP Markers
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三花金钱草 (Desmodium triflorum (L.) DC) 的遗传多样性和地理分化。

DOI:
10.1007/s12374-010-9102-2
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发表时间:
2010-03
影响因子:
2.9
通讯作者:
Xin, Guo-rong
Xin, Guo-rong
中科院分区:
生物学4区
文献类型:
--
作者:
Zhou, Ren-chao;Yue, Mao-feng;Feng, Li;Huang, Ye-lin;Shi, Su-hua;Xin, Guo-rong

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高水平的遗传变异使物种能够适应不断变化的环境,并为植物育种者提供人工选择所需的原材料。利用6对AFLP引物对三花山蚂蝗(Desmodium triflorum(L.)DC.来自华南地区的12个种群。结果表明,该群体具有较高的多态性位点比例(P = 76.16%)和较高的基因多样性(HT = 0.310),表明该群体的遗传多样性较高。triflorum在物种水平上是高的。群体水平的遗传多样性也较高(P = 55.85%,He = 0.230)。居群间的基因分化系数(GST)为0.255,表明虽然大部分遗传多样性存在于居群内,但居群间也存在相当大的分化。AMOVA分析表明,总变异的24.29%在居群间分配(Φ ST = 0.243)。UPGMA聚类分析结果表明,12个居群可分为3个亚群:东部亚群、西部亚群和中南亚群。然而,Mantel检验显示,这些人群之间的地理距离和遗传距离之间没有显着的相关性(r = 0.286,p = 0.983);山区的基因流和人为干扰的障碍可能混淆了这些相关性。本研究为进一步开发利用D.三叶草
High levels of genetic variation enable species to adapt to changing environments and provide plant breeders with the raw materials necessary for artificial selection. In the present study, six AFLP primer pairs were used to assess the genetic diversity of Desmodium triflorum (L.) DC. from 12 populations in South China. A high percentage of polymorphic loci (P = 76.16%) and high total gene diversity (HT = 0.310) were found, indicating that the genetic diversity of D. triflorum is high at the species level. Genetic diversity was also relatively high at the population level (P = 55.85%, He = 0.230). The coefficient of gene differentiation among populations (GST) was 0.255, indicating that while most genetic diversity resided within populations, there was also considerable differentiation among populations. AMOVA also indicated 24.29% of the total variation to be partitioned among populations (ΦST = 0.243). UPGMA clustering analysis based on genetic distances showed that the 12 populations could be separated into three subgroups: an eastern, a western, and a central-southern subgroup. However, a Mantel test revealed no significant correlation (r = 0.286, p = 0.983) between the geographical distances and genetic distances separating these populations; mountain barriers to gene flow and human disturbance may have confounded these correlations. The present study has provided some fundamental genetic data that will be of use in the exploitation of D. triflorum.
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