Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury
Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury
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DOI：
10.3791/60027
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发表时间：
2019-08-01
影响因子：
1.2
通讯作者：
Islam, Mecca R.
中科院分区：
文献类型：
--
作者：
Schwulst, Steven J.;Islam, Mecca R.
疾病控制和伤害预防中心估计，在美国每年有近200万人遭受创伤性脑损伤（TBI）。事实上，TBI是导致超过三分之一与伤害有关的死亡的因素。然而，TBI病理生理学的细胞和分子机制知之甚少。因此，TBI的临床前模型，能够复制的损伤机制有关的TBI在人类患者是一个关键的研究需要。TBI的受控皮质撞击（CCI）模型利用机械装置直接撞击暴露的皮质。虽然没有模型可以完全概括人类患者中TBI的不同损伤模式和异质性，但CCI能够诱导广泛的临床适用的TBI。此外，CCI很容易标准化，使研究人员能够比较不同实验以及不同研究组的结果。以下方案是在TBI的鼠模型中使用市售撞击装置施加严重CCI的详细描述。
The Centers for Disease Control and Injury Prevention estimate that almost 2 million people sustain a traumatic brain injury (TBI) every year in the United States. In fact, TBI is a contributing factor to over a third of all injury-related mortality. Nonetheless, the cellular and molecular mechanisms underlying the pathophysiology of TBI are poorly understood. Thus, preclinical models of TBI capable of replicating the injury mechanisms pertinent to TBI in human patients are a critical research need. The controlled cortical impact (CCI) model of TBI utilizes a mechanical device to directly impact the exposed cortex. While no model can full recapitulate the disparate injury patterns and heterogeneous nature of TBI in human patients, CCI is capable of inducing a wide range of clinically applicable TBI. Furthermore, CCI is easily standardized allowing investigators to compare results across experiments as well as across investigative groups. The following protocol is a detailed description of applying a severe CCI with a commercially available impacting device in a murine model of TBI.