Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
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DOI：
10.1038/nature04332
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发表时间：
2005-12-22
期刊：
影响因子：
64.8
通讯作者：
Denning, DW
中科院分区：
文献类型：
--
作者：
Nierman, WC;Pain, A;Denning, DW
烟曲霉菌是微生物中的例外，既是原发性和机会致病菌，也是主要过敏原（1-3）。它的分生孢子生产是多产的，因此人类呼吸道接触几乎是恒定的（4）。A.烟曲霉是从人类栖息地（5）和蔬菜堆肥堆（6，7）中分离出来的。在免疫功能低下的个体中，侵袭性感染的发生率可高达50%，死亡率通常约为50%（参考文献2）。A.烟曲霉菌和其他空气传播的真菌与免疫系统越来越多地与严重的哮喘和鼻窦炎有关（8）。虽然A.虽然烟曲霉的数量很多，但生物体的基本生物学大多是模糊的。在这里，我们展示了临床分离株Af 293的完整的29.4兆碱基基因组序列，它由8条染色体组成，含有9，926个预测基因。微阵列分析揭示了不同基因组的温度依赖性表达，以及700 A。烟曲霉基因不存在或显着分歧密切相关的有性物种费氏奈氏酵母，其中许多可能有致病性表型的作用。Af 293基因组序列为未来了解这种非凡的真菌提供了无与伦比的资源。
Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen(1-3). Its conidia production is prolific, and so human respiratory tract exposure is almost constant(4). A. fumigatus is isolated from human habitats(5) and vegetable compost heaps(6,7). In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis(8). Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.