AA.AG@Helix.Ends:: A:A and A:G base-pairs at the ends of 16 S and 23 S rRNA helices
AA.AG@Helix.Ends:: A:A and A:G base-pairs at the ends of 16 S and 23 S rRNA helices
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DOI：
10.1006/jmbi.2001.4807
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发表时间：
2001-07-20
影响因子：
5.6
通讯作者：
Gutell, RR
中科院分区：
文献类型：
--
作者：
Elgavish, T;Cannone, JJ;Gutell, RR
研究表明，在PDB数据库和16 S和23 S rRNA比较结构模型中，RNA晶体和NMR结构的螺旋末端经常出现AA和AG对置，而AG对置的G通常位于螺旋的3 '端。此外，这些对立经常是碱基配对，通常在剪切构象，虽然其他构象存在于NMR和晶体结构。这些A：A和A：G碱基对存在于各种结构环境中，包括GNRA四环、E和E样环、同轴堆叠的两个螺旋之间的界面、串联G：A碱基对、U形转弯和腺苷平台。最后，结构的研究，揭示构象重排发生在区域的RNA与AA和AG反对在螺旋的末端，我们认为，这些构象独特的螺旋延伸可能与功能上重要的结构重排。(C)北京：科学出版社.
This study reveals that AA and AG oppositions occur frequently at the ends of helices in RNA crystal and NMR structures in the PDB database and in the 16 S and 23 S rRNA comparative structure models, with the G usually 3 ' to the helix for the AG oppositions. In addition, these oppositions are frequently base-paired and usually in the sheared conformation, although other conformations are present in NMR and crystal structures. These A:A and A:G base-pairs are present in a variety of structural environments, including GNRA tetraloops, E and E-like loops, interfaced between two helices that are coaxially stacked, tandem G:A base-pairs, U-turns, and adenosine platforms. Finally, given structural studies that reveal conformational rearrangements occurring in regions of the RNA with AA and AG oppositions at the ends of helices, we suggest that these conformationally unique helix extensions might be associated with functionally important structural rearrangements. (C) 2001 Academic Press.