The domain structure of centromeres is conserved from fission yeast to humans
The domain structure of centromeres is conserved from fission yeast to humans
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DOI：
10.1091/mbc.12.9.2767
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发表时间：
2001-09-01
影响因子：
3.3
通讯作者：
Ekwall, K
中科院分区：
文献类型：
--
作者：
Kniola, B;O'Toole, E;Ekwall, K
裂殖酵母的着丝粒 DNA 排列有一个中央核心，两侧是重复序列。着丝粒相关蛋白 Mis6p 和 Cnp1p (SpCENP-A) 仅与中央核心 DNA 相关，而 Swi6 蛋白与周围的重复序列结合。在这里，电子显微镜和免疫荧光光学显微镜揭示了中央核心和侧翼区域在异染色质域内占据不同的位置。含有 Ndc80 蛋白的“锚定”结构位于该异染色质结构域和纺锤体极体之间。裂殖酵母中着丝粒相关蛋白的组织让人想起人类动粒的多层结构，表明这种结构域结构在真核生物中是保守的。
The centromeric DNA of fission yeast is arranged with a central core flanked by repeated sequences. The centromere-associated proteins, Mis6p and Cnp1p (SpCENP-A), associate exclusively with central core DNA, whereas the Swi6 protein binds the surrounding repeats. Here, electron microscopy and immunofluorescence light microscopy reveal that the central core and flanking regions occupy distinct positions within a heterochromatic domain. An "anchor" structure containing the Ndc80 protein resides between this heterochromatic domain and the spindle pole body. The organization of centromere-associated proteins in fission yeast is reminiscent of the multilayered structures of human kinetochores, indicating that such domain structure is conserved in eukaryotes.