Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil
Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil
复制标题
柴油生物降解过程中红球菌 LH 的细胞内代谢变化
DOI：
10.1007/s10126-018-9850-4
复制
发表时间：
2018
影响因子：
3
通讯作者：
Li Hao
中科院分区：
文献类型：
--
作者：
Chen Ze;Zheng Zhou;Wang Feng-Lian;Niu Yuan-Pu;Miao Jin-Lai;Li Hao
In recent years, some marine microbes have been used to degrade diesel oil. However, the exact mechanisms underlying the biodegradation are still poorly understood. In this study, a hypothermophilous marine strain, which can degrade diesel oil in cold seawater was isolated from Antarctic floe-ice and identified and named asRhodococcussp. LH. To clarify the biodegradation mechanisms, a gas chromatography-mass spectrometry (GC-MS)-based metabolomics strategy was performed to determine the diesel biodegradation process-associated intracellular biochemical changes inRhodococcussp. LH cells. With the aid of partial least squares-discriminant analysis (PLS-DA), 17 differential metabolites with variable importance in the projection (VIP) value greater than 1 were identified. Results indicated that the biodegradation of diesel oil byRhodococcussp. LH was affected by many different factors.Rhodococcussp. LH could degrade diesel oil through terminal or sub-terminal oxidation reactions, and might also possess the ability to degrade aromatic hydrocarbons. In addition, some surfactants, especially fatty acids, which were secreted byRhodococcusinto medium could also assist the strain in dispersing and absorbing diesel oil. Lack of nitrogen in the seawater would lead to nitrogen starvation, thereby restraining the amino acid circulation inRhodococcussp. LH. Moreover, nitrogen starvation could also promote the conversation of relative excess carbon source to storage materials, such as 1-monolinoleoylglycerol. These results would provide a comprehensive understanding about the complex mechanisms of diesel oil biodegradation byRhodococcussp. LH at the systematic level.
