Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions.
Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions.
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在标准实验室条件下，铜绿假单胞菌向更高适应性进化。
DOI：
10.1038/s41396-020-00841-6
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Häussler S
中科院分区：
文献类型：
--
作者：
Grekov I;Thöming JG;Kordes A;Häussler S
确定致病菌适应性表型进化的遗传因素是理解传染病形成的关键。在这项研究中，我们进行了突变积累实验，以记录突变的频率和它们对环境细菌铜绿假单胞菌的hypermutator菌株的适应性的影响相比，宿主生态位适应的肠道沙门氏菌。我们证明，铜绿假单胞菌，而不是S。enterica，hypermutators进化向更高的健身在缺氧条件下。适应增加的生长性能是伴随着可逆的扰动膜和细胞壁生物合成基因的局部遗传背景。此外，我们观察到一个微调的复杂的监管电路，涉及多个二鸟苷酸调节酶，调节快速增长之间的过渡和固着生物膜相关的生活方式。二鸟苷酸信号传导途径的冗余和局部特异性似乎允许在小生境适应的克隆铜绿假单胞菌谱系中朝向增加的生长性能的趋同转变，这伴随着它们的运动性、毒力和生物膜表型的显著异质性。
Identifying genetic factors that contribute to the evolution of adaptive phenotypes in pathogenic bacteria is key to understanding the establishment of infectious diseases. In this study, we performed mutation accumulation experiments to record the frequency of mutations and their effect on fitness in hypermutator strains of the environmental bacterium Pseudomonas aeruginosa in comparison to the host-niche-adapted Salmonella enterica. We demonstrate that P. aeruginosa, but not S. enterica, hypermutators evolve toward higher fitness under planktonic conditions. Adaptation to increased growth performance was accompanied by a reversible perturbing of the local genetic context of membrane and cell wall biosynthesis genes. Furthermore, we observed a fine-tuning of complex regulatory circuits involving multiple di-guanylate modulating enzymes that regulate the transition between fast growing planktonic and sessile biofilm-associated lifestyles. The redundancy and local specificity of the di-guanylate signaling pathways seem to allow a convergent shift toward increased growth performance across niche-adapted clonal P. aeruginosa lineages, which is accompanied by a pronounced heterogeneity of their motility, virulence, and biofilm phenotypes.
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影响因子：
6.7
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1308523110
发表时间：
2013-08-27
影响因子：
11.1
作者：
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通讯作者：
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64.8
作者：
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