Increase of cytosolic calcium induced by trichosanthin suppresses cAMP/PKC levels through the inhibition of adenylyl cyclase activity in HeLa cells
Increase of cytosolic calcium induced by trichosanthin suppresses cAMP/PKC levels through the inhibition of adenylyl cyclase activity in HeLa cells
复制标题
天花粉素诱导胞浆钙增加，通过抑制 HeLa 细胞中腺苷酸环化酶活性来抑制 cAMP/PKC 水平
DOI：
10.1007/s11033-010-0432-4
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Wang, Ping
中科院分区：
文献类型：
--
作者：
Jiang, Qingsong;Bai, Tumen;Wang, Ping
细胞内游离钙离子浓度升高在天花粉蛋白诱导的细胞凋亡中起关键作用。然而，很少有人知道细胞内钙增加对腺苷酸环化酶活性和细胞内cAMP信号通路的影响在HeLa细胞。本研究表明，细胞外Ca ~（2+）的内流所需的抑制腺苷酸环化酶的活性和细胞内cAMP水平的下降，由天花粉蛋白启动。此外，这种抑制被取消激活的PKC，而不是PKA。因此，我们的研究结果表明，增加细胞内钙诱导的天花粉蛋白通过抑制腺苷酸环化酶活性抑制cAMP水平。
Increase of cytosolic free calcium played a pivotal role in apoptotic cells induced by trichosanthin. However, little is known about the influence of cytosolic calcium increase on adenylyl cyclase activity and intracellular cAMP signaling pathway in HeLa cells. The present study showed that an influx of extracellular Ca2+ initiated by trichosanthin was required for the suppression of adenylyl cyclase activity and decrease of intracellular cAMP level. Furthermore, this inhibition was abolished by activation of PKC rather than PKA. Therefore, our results suggested that increase of cytosolic calcium induced by trichosanthin inhibits cAMP levels via suppression of adenylyl cyclase activity.