Activation of immediate early gene, c-fos, and c-jun in the rat small intestine after ischemia/reperfusion
Activation of immediate early gene, c-fos, and c-jun in the rat small intestine after ischemia/reperfusion
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DOI：
10.1097/00007890-200002270-00022
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发表时间：
2000-02-27
期刊：
影响因子：
6.2
通讯作者：
Miwa, K
中科院分区：
文献类型：
--
作者：
Itoh, H;Yagi, M;Miwa, K
背景在肝、心、肾等器官的缺血/再灌注损伤后，激活的即刻早期基因（IEGs）在介导细胞反应中起着关键作用。然而，目前还没有报道研究IEGs的激活与I/R后细胞再生或程序性细胞死亡之间的关系。我们用逆转录-聚合酶链反应和北方印迹分析检测了大鼠小肠I/R后c-fos和c-jun的顺序表达，并比较了共存的两个参数的模式：（1）通过增殖细胞核抗原的免疫组织化学检测确定的再生，（2）采用末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）和DNA片段化法检测细胞程序性死亡。再灌注15 min，c-fos和c-jun mRNA表达明显增加，分别为对照组的6.3倍和4.4倍。再灌注后5分钟至4小时，心肌细胞核抗原表达显著升高，在30分钟达到高峰。再灌注60 min细胞凋亡达高峰。TUNEL法检测到I/R后吸收上皮细胞核凋亡，免疫组化法检测到c-Fos和c-dun蛋白表达。这些结果表明，在小肠I/R后c-fos和c-jun的过度表达与程序性细胞死亡和随后的细胞再生相关。
Background. Activated immediate early genes (IEGs) play key roles in mediating cellular response after ischemia/reperfusion (I/R) injuries in some organs such as liver, heart and kidney. However, there is no report investigating an association between the activation of IEGs and cellular regeneration or programmed cell death after I/R in small intestine.Methods. We examined a sequential expression of c-fos and c-jun after I/R in rat small intestine using reverse transcription-polymerase chain reaction and Northern blot analysis, and compared the patterns with coexistent two parameters: (1) regeneration determined by immunohistochemical detection of proliferating cell nuclear antigen, (2) programmed cell death determined with the terminal deoxynucleotidyl-transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method and DNA fragmentation.Results. The expression of c-fos and c-jun mRNA increased markedly 15 min after reperfusion and was, respectively, 6.3 and 4.4 times higher than in controls. Proliferating cell nuclear antigen expression was significantly elevated between 5 min and 4 hr, peaking at 30 min after reperfusion. Apoptosis showed a peak 60 min after reperfusion. Apoptosis after I/R was detected in the nuclei of absorptive epithelial cells by the TUNEL method, and these apoptotic signals were consistent with the expression of c-Fos and c-dun proteins using an immunohistochemical method.Conclusions. These results suggest that overexpression of c-fos and c-jun after I/R in the small intestine correlates with programmed cell death and subsequent cellular regeneration.