Protective Effects of Methane-Rich Saline on Renal Ischemic-Reperfusion Injury in a Mouse Model.
Protective Effects of Methane-Rich Saline on Renal Ischemic-Reperfusion Injury in a Mouse Model.
复制标题
富含甲烷的盐水对小鼠模型肾缺血再灌注损伤的保护作用。
DOI：
10.12659/msm.911156
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发表时间：
2018-10-31
期刊：
影响因子：
--
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Meng Y;Jiang Z;Li N;Zhao Z;Cheng T;Yao Y;Wang L;Liu Y;Deng X
背景 肾缺血再灌注（RIR）损伤仍然是急性肾损伤的主要原因，导致院内死亡率和慢性肾病风险增加。先前的研究提出，氧化应激、炎症和肾细胞凋亡是最常见的损伤原因，而最近的研究证明，甲烷是肠道微生物产生或伴随活性氧（ROS）产生的最简单的烷烃，可以减轻炎症和氧化应激，并减少不同器官的细胞凋亡。材料/方法在本研究中，我们分析了富含甲烷的盐水对小鼠模型 RIR 损伤的可能影响，并分析了其对炎症、氧化应激和细胞凋亡的可能保护作用。结果 结果表明，甲烷治疗显着改善了血肌酐和血尿素氮 (BUN) 水平，并改善了 RIR 损伤中的肾脏组织学。进一步的实验证明，这种保护作用主要表现在肾组织氧化应激减少、细胞凋亡减少、炎症减少以及全身反应改善。结论 我们目前的研究证明了甲烷对 RIR 损伤的保护作用，并与之前的研究一起证实了其多器官保护作用。这可能有助于转化甲烷的应用并开发其在器官缺血再灌注损伤中的应用。
Background Renal ischemic-reperfusion (RIR) injury remains a major cause of acute kidney injury, with increased in-hospital mortality and risks for chronic kidney disease. Previous studies have proposed that oxidative stress, inflammation, and renal apoptosis are the most common causes of injury, whereas recent research proved that methane, the simplest alkane generated by an enteric microorganism or accompanying the production of reactive oxygen species (ROS), can alleviate inflammation and oxidative stress and reduce apoptosis in different organs. Material/Methods In the present study, we analyzed the possible effects of methane-rich saline in RIR injury in a mouse model and analyzed its possible protective effects on inflammation, oxidative stress, and apoptosis. Results The results showed that treatment with methane significantly improved blood creatinine and blood urea nitrogen (BUN) levels and improved renal histology in RIR injury. Further experimentation proved that this protective effect was primarily manifested in decreased oxidative stress, less apoptosis, and reduced inflammation in renal tissues, as well as improved general responses. Conclusions Our present study proved the protective effects of methane in RIR injury and, together with previous research, confirmed the multi-organ protective effects. This may help to translate methane application and develop its use in organ ischemic-reperfusion injury.