An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells
An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells
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一种基于纳米材料的高效电化学生物传感器，用于灵敏识别耐药白血病细胞
作者：
Zhang Shaolian;Zhang Lu;Zhang Xi;Yang Peihui;Cai Jiye
基于p -糖蛋白（P-gp）在细胞膜上的表达水平，研制了一种新的电化学细胞传感器，用于耐药白血病K562/ADM细胞的快速、高灵敏度识别。选择纳米金/聚苯胺纳米纤维的纳米复合界面（AuNPs/PANI-NF）设计用于电化学检测的生物传感器。基于Au/ pani - nf的细胞传感器包被抗p -糖蛋白（anti-P-gp）分子，为细胞表面p -糖蛋白的免疫传感提供了仿生界面，从而可以捕获过表达的P-gp细胞。透射电子显微镜（TEM）显示，金纳米粒子沿PANI-NF表面结构均匀锚定，呈现直径约70 nm的纤维状形态，原子力显微镜（AFM）进一步呈现纳米复合膜的形态。由于抗p -gp对白血病K562/ADM细胞具有较高的亲和力，因此该传感器对白血病K562/ADM细胞的检测范围为1.6 × 102 ~ 1.6 × 106细胞/ mL，检测限为80个细胞/ mL，回收率实验表明灵敏度适合实际应用。流式细胞术实验分析了细胞表面P-gp的表达水平，证实了上述识别结果。该策略也是一种成本效益高、操作方便的方法，对癌细胞和细胞表面受体的敏感识别具有很大的前景；因此，它有助于癌症的诊断。
A novel electrochemical cytosensor was developed for the fast and high-sensitivity recognition of drug-resistant leukemia K562/ADM cells based on the P-glycoprotein (P-gp) expression level on a cell membrane. The nanocomposite interface of the gold nanoparticles/polyaniline nanofibers (AuNPs/PANI-NF) was chosen to design the biosensor for electrochemical detection. Au/PANI-NF-based cytosensors coated with anti-P-glycoprotein (anti-P-gp) molecules could provide a biomimetic interface for the immunosensing of cell surface P-glycoprotein, and thus could capture the over-expression P-gp cells. Transmission electron microscopy (TEM) indicated that the gold nanoparticles were uniformly anchored along the structure of the PANI-NF surface, displaying fibrillar morphology with a diameter of ∼70 nm, and atomic force microscopy (AFM) further presented the morphology of the nanocomposite film. Owing to the high affinity of anti-P-gp for leukemia K562/ADM cells of the propounded sensing platform, the proposed biosensor exhibited excellent analytical performance for leukemia K562/ADM cells, ranging from 1.6 × 102 to 1.6 × 106 cells per mL with a detection limit of 80 cells per mL. Recovery experiments indicated that the sensitivity reported here is suitable for practical application. The cell surface P-gp expression level was analysed by flow cytometric experiments, which confirmed the above recognized result. This strategy is also a cost-effective and convenient operation, implying great promise for the sensitive recognition of cancer cells and cell surface receptors; thus, it is helpful in cancer diagnosis.