Expression Profiling of Tobacco Leaf Trichomes Identifies Genes for Biotic and Abiotic Stresses
Expression Profiling of Tobacco Leaf Trichomes Identifies Genes for Biotic and Abiotic Stresses
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DOI：
10.1093/pcp/pcq118
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发表时间：
2010-10-01
影响因子：
4.9
通讯作者：
Choi, Yong-Eui
中科院分区：
文献类型：
--
作者：
Harada, Emiko;Kim, Ji-Ah;Choi, Yong-Eui
烟草植株有短腺毛和长腺毛。有证据表明，烟草毛状体在抵御生物和非生物胁迫方面发挥着多种作用。从对照和镉(Cd)处理的叶毛中构建了cDNA文库。对近2000个表达序列标签(EST)基因克隆进行了测序，以分析对照和Cd处理的叶毛中的基因表达。应激反应和初级新陈代谢的基因得分很高，这表明毛状体是一个具有生物活性和应激反应的组织。逆转录-聚合酶链式反应(RT-PCR)分析表明，抗病原T-叶轮藻素样蛋白、谷胱甘肽过氧化物酶和几类病程相关(PR)蛋白在毛状体中特异或主要表达。富含半胱氨酸的PR蛋白，如非特异性脂转移蛋白(NsLTP)和金属羧基肽酶抑制剂，可用于金属的封存。Cd处理诱导叶片和毛状体中渗透素和类thaumatin蛋白的表达。激光共聚焦扫描显微镜(CLSM)显示，长毛和短毛顶端细胞中的谷胱甘肽含量均高于其他类型的叶细胞，表明毛状体中存在一种活性的硫依赖保护系统。我们的结果表明，毛状体特异转录组方法是研究毛状体对非生物和生物胁迫的防御功能的有力工具。毛状体是一种丰富的有用基因来源，可用于耐逆植物的分子育种。
Nicotiana tabacum (tobacco) plants have short and long glandular trichomes. There is evidence that tobacco trichomes play several roles in the defense against biotic and abiotic stresses. cDNA libraries were constructed from control and cadmium (Cd)-treated leaf trichomes. Almost 2,000 expressed sequence tag (EST) cDNA clones were sequenced to analyze gene expression in control and Cd-treated leaf trichomes. Genes for stress response as well as for primary metabolism scored highly, indicating that the trichome is a biologically active and stress-responsive tissue. Reverse transcription-PCR (RT-PCR) analysis demonstrated that antipathogenic T-phylloplanin-like proteins, glutathione peroxidase and several classes of pathogenesis-related (PR) proteins were expressed specifically or dominantly in trichomes. Cysteine-rich PR proteins, such as non-specific lipid transfer proteins (nsLTPs) and metallocarboxypeptidase inhibitors, are candidates for the sequestration of metals. The expression of osmotin and thaumatin-like proteins was induced by Cd treatment in both leaves and trichomes. Confocal laser scanning microscopy (CLSM) showed that glutathione levels in tip cells of both long and short trichomes were higher than those in other types of leaf cells, indicating the presence of an active sulfur-dependent protective system in trichomes. Our results revealed that the trichome-specific transcriptome approach is a powerful tool to investigate the defensive functions of trichomes against both abiotic and biotic stress. Trichomes are shown to be an enriched source of useful genes for molecular breeding towards stress-tolerant plants.