The 30-Kilodalton Protein Present in Purified Fusicoccin Receptor Preparations Is a 14-3-3-Like Protein
The 30-Kilodalton Protein Present in Purified Fusicoccin Receptor Preparations Is a 14-3-3-Like Protein
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纯化的梭菌素受体制剂中存在的 30 千道尔顿蛋白质是一种 14-3-3 样蛋白质
DOI：
10.1104/pp.106.4.1497
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
P. Aducci
中科院分区：
文献类型：
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作者：
M. Marra;M. R. Fullone;Vincenzo Fogliano;Jan Pen;Maurizio Mattei;Serena Masi;P. Aducci
我们最近报道了从玉米（Zea mays L.）以及通过光亲和标记对其进行鉴定（P. Aducci，A. Ballio，V. Fogliano，M.R. Fullone，M. Marra，N. Proietti [1993] Eur J Biochem 214：339-345）。FC受体的纯制剂，在非变性条件下获得的，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示两个双峰的蛋白质的表观分子量为30和90 kD。在本文中，我们描述了分离和鉴定的30 kD的双联体蛋白的一级结构。对消化30 kD蛋白质所得肽的测序研究显示，与来自玉米的14-3- 3样蛋白（命名为GF 14）完全相同。14-3-3家族是一类广泛分布于真核生物中的蛋白质，已知其发挥多种调节作用。30-kD蛋白质已经通过针对基于确定的氨基酸序列的合成肽制备的特异性抗体进行免疫学鉴定。一个类似的蛋白质被确认在部分纯化的FC受体制剂从豆和菠菜叶。
We have recently reported on the purification of the fusicoccin (FC) receptor from corn (Zea mays L.) and its identification by photoaffinity labeling (P. Aducci, A. Ballio, V. Fogliano, M.R. Fullone, M. Marra, N. Proietti [1993] Eur J Biochem 214: 339–345). Pure preparations of FC receptors, obtained under nondenaturing conditions, showed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis two doublets of proteins with apparent molecular masses of 30 and 90 kD. In the present paper we describe the isolation and identification of the primary structure of the 30-kD doublet proteins. Sequencing studies of peptides resulting from the digestion of the 30-kD protein showed a full identity with a 14–3–3like protein from corn, named GF14. The 14–3–3 family is a class of proteins that is widely distributed in eukaryotes and is known to play various regulatory roles. The 30-kD protein has been immunologically identified by specific antibodies prepared against a synthetic peptide based on the determined amino acid sequence. A similar protein is recognized in partially purified FC receptor preparations from bean and spinach leaves.