Inherent Charge-Shifting Polyelectrolyte Multilayer Blends: A Facile Route for Tunable Protein Release from Surfaces
Inherent Charge-Shifting Polyelectrolyte Multilayer Blends: A Facile Route for Tunable Protein Release from Surfaces
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DOI：
10.1021/bm200566k
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发表时间：
2011-08-01
影响因子：
6.2
通讯作者：
Hammond, Paula T.
中科院分区：
文献类型：
--
作者：
Hong, Jinkee;Kim, Byeong-Su;Hammond, Paula T.
最近的研究强调了可降解的多层膜，使不同的治疗药物的程序释放。可以进行拆卸的分层沉积（LbL）构建的多层膜已被证明是相当有趣的，特别是生物医学表面涂层，因为它们的多功能性和温和的水处理条件，能够包含具有高活性的生物药物。在这项研究中，我们研究了一种蛋白质的控制释放，使用不同的膜分解机制，在释放条件下膜相互作用的逐渐解离。聚（β -氨基酯）s和聚（l -赖氨酸）（PLL）作为带正电的多层组分，与带负电的模型抗原蛋白卵清蛋白（Ova）共组装。蛋白质从这些多层膜的释放主要是在生理pH条件下组分电荷的缓慢变化，而不是水解降解释放。通过改变沉积溶液中两种多胺的比例，释放的时间尺度可以变化近2个数量级。这些薄膜的高度通用性和可调特性为设计使用各种多离子的药物涂层的可控和顺序递送奠定了基础。
Recent research has highlighted degradable multilayer films that enable the programmed release of different therapeutics. Multilayers constructed by the layer-by-layer (LbL) deposition that can undergo disassembly have been demonstrated to be of considerable interest, particularly for biomedical surface coatings due to their versatility and mild aqueous processing conditions, enabling the inclusion of biologic drugs with high activity. In this study, we examine the controlled release of a protein using a different mechanism for film disassembly, the gradual dissociation of film interactions under release conditions. Poly(beta-amino ester)s and poly(L-lysine) (PLL) were used as the positively charged multilayer components coassembled with a model negatively charged antigen protein, ovalbumin (Ova). The release of the protein from these multilayer films is dominated by the slow shift in the charge of components under physiological pH conditions rather than by hydrolytic degradative release. The time scale of release can be varied over almost 2 orders of magnitude by varying the ratio of the two polyamines in the deposition solution. The highly versatile and tunable properties of these films form a basis for designing controlled and sequential delivery of drug coatings using a variety of polyions.