Methods for voltage-sensitive dye imaging of rat cortical activity with high signal-to-noise ratio
Methods for voltage-sensitive dye imaging of rat cortical activity with high signal-to-noise ratio
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DOI：
10.1152/jn.01169.2006
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发表时间：
2007-07-01
影响因子：
2.5
通讯作者：
Wu, Jian-Young
中科院分区：
文献类型：
--
作者：
Lippert, Michael T.;Takagaki, Kentaroh;Wu, Jian-Young
高信噪比下大鼠大脑皮层活动的电压敏感染料成像方法。J Neurophysiol 98：502-512，2007.首次发表于2007年5月9日; doi：10.1152/ jn.01169.2006。我们描述的方法，以实现高灵敏度的电压敏感染料（VSD）成像从大鼠桶和视觉皮层在体内与使用的蓝色染料RH 1691和高动态范围的成像设备（光电二极管阵列）。通过改进染色方案和离线程序去除脉动伪影，VSD记录的灵敏度与来自相同位置的局部场电位记录的灵敏度相当。利用这种灵敏度，可以从类似于500个单独的探测器记录，每个探测器覆盖直径为160 μ m的皮层组织区域（总成像场类似于直径为4 mm），时间分辨率为1，600帧/秒，而无需多次试验平均。我们可以记录80-100次试验的间歇性10秒试验从每个成像领域之前，室间隔缺损信号减少到一半的初始幅度，因为漂白和洗出。两者合计，本报告中描述的方法提供了一个有用的工具，可视化诱发和自发波从啮齿动物皮层。
Methods for voltage-sensitive dye imaging of rat cortical activity with high signaltonoise ratio. J Neurophysiol 98: 502-512, 2007. First published May 9, 2007; doi: 10.1152/ jn.01169.2006. We describe methods to achieve high sensitivity in voltage-sensitive dye (VSD) imaging from rat barrel and visual cortices in vivo with the use of a blue dye RH1691 and a high dynamic range imaging device (photodiode array). With an improved staining protocol and an off-line procedure to remove pulsation artifact, the sensitivity of VSD recording is comparable with that of local field potential recording from the same location. With this sensitivity, one can record from similar to 500 individual detectors, each covering an area of cortical tissue 160 mu m in diameter (total imaging field similar to 4 mm in diameter) and a temporal resolution of 1,600 frames/s, without multiple- trial averaging. We can record 80-100 trials of intermittent 10-s trials from each imaging field before the VSD signal reduces to one half of its initial amplitude because of bleaching and wash-out. Taken together, the methods described in this report provide a useful tool for visualizing evoked and spontaneous waves from rodent cortex.