Evaluation in mice of some iodinated melanoma imaging agents using cryosectioning and multi-wire proportional counting
Evaluation in mice of some iodinated melanoma imaging agents using cryosectioning and multi-wire proportional counting
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DOI：
10.1007/s002590050416
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发表时间：
1999-04
期刊：
影响因子：
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通讯作者：
P. Labarre;J. Papon;M. Moreau;N. Moins;A. Veyre;J. Madelmont
中科院分区：
文献类型：
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作者：
P. Labarre;J. Papon;M. Moreau;N. Moins;A. Veyre;J. Madelmont
AMBIS 4000是一种多线比例计数器，用于碘-125的测量。该检测器在很宽的动态范围内显示线性响应。利用小鼠全身冷冻切片，AMBIS 4000检测器测量的每面积计数率（cpm/mm2）与NaI（Tl）闪烁检测器测量的每克计数率（dpm/g）之间可以建立线性关系。这样就可以构造出校准曲线。这种新方法可以直接直观和定量地评价短系列125i标记苯酰胺在黑色素瘤小鼠中的生物分布。所研究的化合物均表现出良好的肿瘤靶向能力。其中，正- n -（2-二乙基氨基乙基）-4-碘苯甲酰胺给药后，肝脏和肺的摄食量迅速下降，使其成为恶性黑色素瘤的合适示踪剂。
AMBIS 4000, a multi-wire proportional counter, was calibrated for iodine-125 measurements. The detector displayed a linear response over a wide dynamic range. Using whole-body mice cryosections, a linear relationship could be established between count rate per area (cpm/mm2) measured with the AMBIS 4000 detector and the count rate per gram (dpm/g) determined with an NaI(Tl) scintillation detector. A calibration curve could, thus be constructed. This new method allowed direct visual and quantitative evaluation of the biodistribution of a short series of125I-labelled benzamides in melanoma-bearing mice. All the compounds studied showed good tumoral targeting ability. For one of them, ortho-N-(2-diethylaminoethyl)-4-iodobenzamide, liver and lung uptake decreased rapidly after dosing, making it a suitable tracer for scintigraphic detection of malignant melanoma.